Comprehensive analysis of ankyrin repeat gene family revealed SbANK56 confers drought tolerance in sorghum

IF 4.5 2区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental and Experimental Botany Pub Date : 2024-10-02 DOI:10.1016/j.envexpbot.2024.105989

Juanying Zhao , Huiyan Wang , Yujie Wu , Jun Yang , Xinqi Fan , Du Liang , Xiaojuan Zhang , Qi Guo , Qingshan Liu , Yizhong Zhang

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引用次数: 0

Abstract

Ankyrin repeat (ANK) proteins are crucial for cell growth, development, and response to hormones and environmental stress. However, there has been little research done to clarify the roles of ANK proteins in sorghum. In this study, 142 ANK genes of sorghum were identified and classified into 12 subfamilies according to the conserved domains. The cis-elements analysis revealed a substantial presence of stress-responsive elements within the promoter region of SbANK genes. After treated with drought, salt, and abscisic acid, SbANK56 showed the highest expression levels among family members by using quantitative real-time PCR (qRT-PCR) analysis. The survival rate was significantly improved by the overexpression of SbANK56 compared to wild type (WT) under drought conditions. SbANK56 overexpressing plants displayed lower malondialdehyde and higher proline contents compared to WT plants under drought conditions. Additionally, the expression levels of drought-associated genes were significantly increased in SbANK56 transgenic plants. Importantly, the analysis of natural variation in SbANK56 revealed a significant positive correlation between SbANK56_Hap4 and both its differential expression and drought stress tolerance. Taken together, our results provide some evidence for improving drought tolerance in sorghum through breeding initiatives while also advancing our knowledge of the evolutionary trends and functional mechanisms underlying ANK genes.

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全面分析杏仁蛋白重复基因家族发现 SbANK56 能赋予高粱耐旱性

安基蛋白重复序列（ANK）蛋白对细胞的生长、发育以及对激素和环境压力的反应至关重要。然而，关于 ANK 蛋白在高粱中的作用的研究却很少。本研究鉴定了 142 个高粱 ANK 基因，并根据保守结构域将其分为 12 个亚族。顺式元件分析表明，在SbANK基因的启动子区域存在大量胁迫响应元件。通过实时定量 PCR（qRT-PCR）分析，SbANK56 在干旱、盐分和脱落酸处理后的表达水平在家族成员中最高。与野生型（WT）相比，过表达 SbANK56 能明显提高干旱条件下的存活率。在干旱条件下，与 WT 植物相比，过表达 SbANK56 的植物丙二醛含量更低，脯氨酸含量更高。此外，SbANK56 转基因植株中干旱相关基因的表达水平也显著增加。重要的是，对 SbANK56 自然变异的分析表明，SbANK56Hap4 与其差异表达和干旱胁迫耐受性之间存在明显的正相关。总之，我们的研究结果为通过育种措施提高高粱的抗旱性提供了一些证据，同时也增进了我们对 ANK 基因的进化趋势和功能机制的了解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental and Experimental Botany 环境科学-环境科学

CiteScore

9.30

自引率

5.30%

发文量

342

审稿时长

26 days

期刊介绍： Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment. In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief. The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB. The areas covered by the Journal include: (1) Responses of plants to heavy metals and pollutants (2) Plant/water interactions (salinity, drought, flooding) (3) Responses of plants to radiations ranging from UV-B to infrared (4) Plant/atmosphere relations (ozone, CO2 , temperature) (5) Global change impacts on plant ecophysiology (6) Biotic interactions involving environmental factors.