Olive mill wastewater as a source of defense-promoting by-products against microbial pathogens

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2024-10-05 DOI:10.1016/j.stress.2024.100623

Ascenzo Salvati , Fabio Sciubba , Alessandra Diomaiuti , Gian Paolo Leone , Daniele Pizzichini , Daniela Bellincampi , Daniela Pontiggia

{"title":"Olive mill wastewater as a source of defense-promoting by-products against microbial pathogens","authors":"Ascenzo Salvati , Fabio Sciubba , Alessandra Diomaiuti , Gian Paolo Leone , Daniele Pizzichini , Daniela Bellincampi , Daniela Pontiggia","doi":"10.1016/j.stress.2024.100623","DOIUrl":null,"url":null,"abstract":"<div><div>Olive oil is a core component of the Mediterranean diet known for its nutritional properties and health benefits. Olive industry is moving to novel extraction systems for higher oil yield and quality and for waste reduction, which is a relevant problem in the process due to its toxicity and high disposal costs. Multi-Phase Decanter (DMF) is a modern two-phase system performed without adding water during the process. Using DMF, a wet by-product indicated as pâté and consisting of the fruit pulp and vegetation water (VW) is recovered. The pâté has a high content of potentially bioactive molecules that may be exploited to promote plant resistance against microbial pathogens. In this work, to identify by/products of biological interest, the VW recovered from the pâté by centrifugation was subjected to fractionation by tangential-flow membrane filtration (TFMF), combining microfiltration (MF) and ultrafiltration (UF). High-resolution NMR spectroscopy indicated the presence of bioactive molecules such as flavonoids, hydroxytyrosol and oleuropein with known antimicrobial activity. High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) was performed to detect the presence of pectic oligosaccharides in the fractions, showing the enrichment, in the UF-concentrate fraction, of oligogalacturonides (OGs), well known for the ability to elicit defense responses and protect plants against pathogen infections. <em>Arabidopsis thaliana</em> plants treated with TFMF fractions displayed induction of defense responses and exhibited tolerance against microbial pathogens without adverse effects on growth and fitness. This study shows that pâté by-products can potentially be exploited in agriculture as sustainable plant phyto-protectant.</div></div>","PeriodicalId":34736,"journal":{"name":"Plant Stress","volume":"14 ","pages":"Article 100623"},"PeriodicalIF":6.8000,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Stress","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667064X24002768","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Olive oil is a core component of the Mediterranean diet known for its nutritional properties and health benefits. Olive industry is moving to novel extraction systems for higher oil yield and quality and for waste reduction, which is a relevant problem in the process due to its toxicity and high disposal costs. Multi-Phase Decanter (DMF) is a modern two-phase system performed without adding water during the process. Using DMF, a wet by-product indicated as pâté and consisting of the fruit pulp and vegetation water (VW) is recovered. The pâté has a high content of potentially bioactive molecules that may be exploited to promote plant resistance against microbial pathogens. In this work, to identify by/products of biological interest, the VW recovered from the pâté by centrifugation was subjected to fractionation by tangential-flow membrane filtration (TFMF), combining microfiltration (MF) and ultrafiltration (UF). High-resolution NMR spectroscopy indicated the presence of bioactive molecules such as flavonoids, hydroxytyrosol and oleuropein with known antimicrobial activity. High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) was performed to detect the presence of pectic oligosaccharides in the fractions, showing the enrichment, in the UF-concentrate fraction, of oligogalacturonides (OGs), well known for the ability to elicit defense responses and protect plants against pathogen infections. Arabidopsis thaliana plants treated with TFMF fractions displayed induction of defense responses and exhibited tolerance against microbial pathogens without adverse effects on growth and fitness. This study shows that pâté by-products can potentially be exploited in agriculture as sustainable plant phyto-protectant.

Abstract Image

查看原文本刊更多论文

作为抗微生物病原体的防御促进副产品来源的橄榄油厂废水

橄榄油是地中海饮食的核心成分，以其营养和保健功效而闻名。为了提高橄榄油的产量和质量，减少废弃物，橄榄油行业正在向新型萃取系统转变，而废弃物因其毒性和高昂的处理成本而成为萃取过程中的一个相关问题。多相卧螺沉降离心机（DMF）是一种现代化的两相系统，在加工过程中无需加水。通过使用 DMF，可回收由果肉和植被水（VW）组成的湿副产品，即肉酱。这种果酱含有大量潜在的生物活性分子，可用于提高植物对微生物病原体的抵抗力。在这项工作中，为了确定具有生物活性的副产品/产物，采用切向流膜过滤（TFMF）技术，结合微滤（MF）和超滤（UF）技术，对通过离心分离从果馅中回收的果汁进行分馏。高分辨率核磁共振光谱显示，其中存在生物活性分子，如黄酮类化合物、羟基酪醇和油菜素，它们具有已知的抗菌活性。为检测馏分中果胶低聚糖的存在，采用了带脉冲安培检测（HPAEC-PAD）的高效阴离子交换色谱法，结果表明在 UF 浓缩馏分中富含寡聚半乳糖苷（OGs），众所周知，寡聚半乳糖苷具有激发防御反应和保护植物免受病原体感染的能力。用 TFMF 部分处理过的拟南芥植物显示出诱导防御反应的能力，并表现出对微生物病原体的耐受性，而不会对生长和健康产生不利影响。这项研究表明，肉酱副产品有可能在农业中用作可持续的植物保护剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.