Microfluidic device with three-dimensional microtip electrodes for efficient capture and concentration of bacteria-sized microparticles using dielectrophoresis
IF 4.1 3区 工程技术Q2 ENGINEERING, ELECTRICAL & ELECTRONIC
Young-Ho Nam , Seok-Young Lee , Seung-Ki Lee , Jong-Ho Kim , Jae-Hyoung Park
{"title":"Microfluidic device with three-dimensional microtip electrodes for efficient capture and concentration of bacteria-sized microparticles using dielectrophoresis","authors":"Young-Ho Nam , Seok-Young Lee , Seung-Ki Lee , Jong-Ho Kim , Jae-Hyoung Park","doi":"10.1016/j.sna.2024.115957","DOIUrl":null,"url":null,"abstract":"<div><div>Microfluidics-based systems have gained considerable attention in the lab-on-chip field owing to their ability to separate, concentrate, and analyze microparticles. Concentrating microparticles is crucial for the high-sensitivity measurement of biomarkers in the analysis of cells or bacteria. This study presents a microfluidic chip using dielectrophoresis (DEP) to capture bacterial microparticles. The chip features a vertically arranged microtip electrode and an indium tin oxide (ITO) electrode, enhancing the electric field concentration effect and enabling optical analysis of the collected particles. The device was designed and fabricated using microfabrication techniques that incorporate a patterned array of microtip electrodes on a polydimethylsiloxane (PDMS) substrate. Experimental studies and numerical simulations were conducted to evaluate the device performance. The fabricated device was applied to the concentration of fluorescent beads with various variables such as particle size, frequency, voltage, and flow rate. The experimental results demonstrated the successful trapping and concentration of microparticles using DEP forces. The recovery rates of the 2.29 µm and 4.42 µm PS beads, when introduced at a flow rate of 1 μL/min and subjected to an applied alternating current (AC) voltage of 200 kHz and 10 V<sub>pp</sub> at the microtip electrode, were measured to be 85.50±2.69 % and 91.83±0.63 %, respectively. Additionally, to assess the applicability of the microtip electrode-based DEP device proposed here for bacteria concentration, capture experiments were conducted using <em>Escherichia coli</em>, demonstrating a recovery rate performance of 77.93±7.31 %. These findings highlight the potential of the proposed microfluidic chip for the concentration and measurement of bacteria, such as <em>E. coli</em>.</div></div>","PeriodicalId":21689,"journal":{"name":"Sensors and Actuators A-physical","volume":"379 ","pages":"Article 115957"},"PeriodicalIF":4.1000,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators A-physical","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0924424724009518","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
Abstract
Microfluidics-based systems have gained considerable attention in the lab-on-chip field owing to their ability to separate, concentrate, and analyze microparticles. Concentrating microparticles is crucial for the high-sensitivity measurement of biomarkers in the analysis of cells or bacteria. This study presents a microfluidic chip using dielectrophoresis (DEP) to capture bacterial microparticles. The chip features a vertically arranged microtip electrode and an indium tin oxide (ITO) electrode, enhancing the electric field concentration effect and enabling optical analysis of the collected particles. The device was designed and fabricated using microfabrication techniques that incorporate a patterned array of microtip electrodes on a polydimethylsiloxane (PDMS) substrate. Experimental studies and numerical simulations were conducted to evaluate the device performance. The fabricated device was applied to the concentration of fluorescent beads with various variables such as particle size, frequency, voltage, and flow rate. The experimental results demonstrated the successful trapping and concentration of microparticles using DEP forces. The recovery rates of the 2.29 µm and 4.42 µm PS beads, when introduced at a flow rate of 1 μL/min and subjected to an applied alternating current (AC) voltage of 200 kHz and 10 Vpp at the microtip electrode, were measured to be 85.50±2.69 % and 91.83±0.63 %, respectively. Additionally, to assess the applicability of the microtip electrode-based DEP device proposed here for bacteria concentration, capture experiments were conducted using Escherichia coli, demonstrating a recovery rate performance of 77.93±7.31 %. These findings highlight the potential of the proposed microfluidic chip for the concentration and measurement of bacteria, such as E. coli.
期刊介绍:
Sensors and Actuators A: Physical brings together multidisciplinary interests in one journal entirely devoted to disseminating information on all aspects of research and development of solid-state devices for transducing physical signals. Sensors and Actuators A: Physical regularly publishes original papers, letters to the Editors and from time to time invited review articles within the following device areas:
• Fundamentals and Physics, such as: classification of effects, physical effects, measurement theory, modelling of sensors, measurement standards, measurement errors, units and constants, time and frequency measurement. Modeling papers should bring new modeling techniques to the field and be supported by experimental results.
• Materials and their Processing, such as: piezoelectric materials, polymers, metal oxides, III-V and II-VI semiconductors, thick and thin films, optical glass fibres, amorphous, polycrystalline and monocrystalline silicon.
• Optoelectronic sensors, such as: photovoltaic diodes, photoconductors, photodiodes, phototransistors, positron-sensitive photodetectors, optoisolators, photodiode arrays, charge-coupled devices, light-emitting diodes, injection lasers and liquid-crystal displays.
• Mechanical sensors, such as: metallic, thin-film and semiconductor strain gauges, diffused silicon pressure sensors, silicon accelerometers, solid-state displacement transducers, piezo junction devices, piezoelectric field-effect transducers (PiFETs), tunnel-diode strain sensors, surface acoustic wave devices, silicon micromechanical switches, solid-state flow meters and electronic flow controllers.
Etc...