Antiaging synergistic effect in noninvasive transdermal delivery of peptide loaded liposomes by low energy/frequency radiofrequency

IF 5.2 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Nanxi Xiang , Zeting Huang , Chunqiao Zhang , Jiahong Huang , Zhenyuan Wang , Jichuan Zhang , Chengyu Wu , Weihua Peng , Jiaheng Zhang
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Abstract

Low energy/frequency radiofrequency (LRF) combined with the transdermal delivery of liposome (L) encapsulated antiaging peptides technology is a remarkable, newly developed physical noninvasive transdermal penetration technique; it is considered a highly efficient, comprehensive and safe technology. In this study, our objective was to evaluate the physical and chemical mechanisms underlying the efficacy of this innovative technique involving a combination of LRF and L, termed LLRF, that exerts a synergistic anti-aging effect on human skin, via an animal experiment. Physical and chemical analyses indicated that a relatively stable liposome with a uniform nano-size, which was formed, possessed good transdermal permeability that was 2.74 folds higher than that of the free peptide (F). LLRF exhibited a higher transdermal permeation performance that was of 3.65 folds higher than that of the free one, which was substantiated via confocal laser scanning fluorescence microscopy. The mouse UVB photoaging model trial confirmed that the LLRF technology exerted a significant synergistic effect compared to liposome technology, or free peptide, by downregulating inflammatory factors (IL-6, TNF-α), inhibiting the mRNA and protein expression of matrix metalloproteinases (MMP1, MMP3), promoting the mRNA and protein expression of related collagens (Procollagen, Col1α1 and Col3α1), and repairing the stratum corneum barrier function, as evidenced by trans-epidermal water loss (TEWL), skin cuticle hydration (SCH), and decreased expression of β-gal, an aging marker. These findings indicated that photoaging skin can be effectively and comprehensively rejuvenated, and that even photodamage can be reversed, thereby restoring the original physiological characteristics of healthy skin. Clinical tests have confirmed that although liposome technology is an effective antiaging method which helps exert tightening and anti-wrinkle effects on human skin, LLRF is an even more effective anti-aging technique. This study reveals a highly effective technique involving a combination physical and chemical therapy that may be utilized for antiaging purposes as well as repairing lightly damaged skin, and can be made readily available in the future.

Abstract Image

低能量/高频射频非侵入性透皮给药多肽脂质体的抗衰老协同效应
低能/高频射频(LRF)结合脂质体(L)包裹抗衰老肽透皮给药技术是一种新开发的非侵入性物理透皮渗透技术,被认为是一种高效、全面和安全的技术。在本研究中,我们的目标是通过动物实验来评估这种创新技术的物理和化学机制,包括 LRF 和 L(称为 LLRF)的组合,它能对人体皮肤产生协同抗衰老效果。理化分析表明,形成的相对稳定的纳米级脂质体具有良好的透皮渗透性,比游离肽(F)的透皮渗透性高 2.74 倍。LLRF 的透皮渗透性比游离肽高出 3.65 倍,这一点已通过共聚焦激光扫描荧光显微镜得到证实。小鼠 UVB 光老化模型试验证实,与脂质体技术或游离肽相比,LLRF 技术具有显著的协同效应,能下调炎症因子(IL-6、TNF-α),抑制基质金属蛋白酶(MMP1、MMP3),促进相关胶原蛋白(Procollagen、Col1α1 和 Col3α1)的 mRNA 和蛋白表达,修复角质层屏障功能,具体表现为经表皮失水(TEWL)、皮肤角质层水合(SCH)和衰老标志物 β-gal 的表达减少。这些研究结果表明,光老化皮肤可以有效、全面地恢复青春活力,甚至可以逆转光损伤,从而恢复健康皮肤原有的生理特征。临床试验证实,虽然脂质体技术是一种有效的抗衰老方法,能帮助人体皮肤发挥紧致和抗皱效果,但 LLRF 是一种更有效的抗衰老技术。这项研究揭示了一种结合物理和化学疗法的高效技术,可用于抗衰老和修复轻度受损的皮肤,并可在未来随时投入使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Pharmaceutics: X
International Journal of Pharmaceutics: X Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
6.60
自引率
0.00%
发文量
32
审稿时长
24 days
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