Biocontrol potential of Bevibacillus brevis HK544 for fungal plant diseases

Abstract

Chemical fungicides have been widely used to control plant diseases caused by fungal pathogens. Still, the overuse of these fungicides has led to concerns about the hazards to humans, animals, and the environment and an increase in fungicide resistance. Interest in biocontrol agents with strong antimicrobial activity has significantly increased to counteract the escalating risks of chemical fungicides. Our previous study found that a soil-born microbe, Brevibacillus brevis HK544, exhibited a promising antifungal activity against a plant pathogen, Fusarium graminearum, with edeine B₁ identified as the active compound. Herein, we extended our study to investigate the in vitro and in vivo antifungal spectrum of B. brevis HK544, including edeine B₁, against various plant pathogenic fungi and evaluated the possibility that B. brevis HK544 could be used in combination with chemical fungicides to enhance the disease control efficacy. With an in vitro broad-spectrum antifungal activity, the B. brevis HK544 culture filtrate, culture broth, and metabolite edeine B₁ exhibited a promising disease control efficacy against tomato gray mold and tomato late blight among the tested fungal diseases. Based on the in vitro antifungal activity against Botrytis cinerea, when edeine B₁ was combined with various chemical fungicides, the highest average synergy score among the tested combinations was observed in the edeine B₁ and benomyl combination. The co-application of the B. brevis HK544 culture filtrate and benomyl improved the disease control efficacy against gray mold disease, supporting the in vitro result. Taken together, our results show that B. brevis HK544 has potential as a biological control agent for controlling plant pathogenic fungi and also that B. brevis HK544 could be combined with chemical fungicides to enhance the efficacy of antifungal agents.