The dynamic and diverse volatile profiles provide new insight into the infective characteristics of Penicillium expansum in postharvest fruit

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-10-12 DOI:10.1016/j.postharvbio.2024.113256

Yuan Wang , Tianyu Chu , Jingxuan Zhang , Danying Ma , Hao Hui , Ivan Kurtovic , Qinglin Sheng , Yahong Yuan , Tianli Yue , Kewei Feng

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引用次数: 0

Abstract

Penicillium expansum causes postharvest disease, fruit loss and patulin (PAT) accumulation in a broad host range and is responsible for considerable health risks and economic losses. In this study, the dynamic volatile organic compounds (VOCs), fungal growth, and PAT accumulation were investigated in apple, pear, citrus and tomato. These indicators revealed the response specificity of fruit to P. expansum attack. PAT content ranged from 2.54 mg/kg to 4.48 mg/kg, while the highest accumulation was detected in citrus. A total of 247 VOCs were identified using GC-IMS and HS-SPME-GC/MS, esters were the most abundant components, followed by alcohols, aldehydes, and ketones. OPLS-DA analysis revealed that the different infection stages were classified successfully. Furthermore, 21, 23, 19, 34 VOCs from each fruit showed significant correlations with PAT accumulation, respectively. Isovaleric acid and 1-hexanol were identified as potential biomarkers in all the fruit. Taken together, P. expansum inoculation caused decay and PAT accumulation in fruit under suitable conditions, leading to a change in VOCs during storage and release of specific volatiles. The present work lays the foundations for studying the interaction mechanism between P. expansum and host, as well as early detection of P. expansum infection and PAT accumulation in postharvest fruit.

查看原文本刊更多论文

动态和多样化的挥发性特征为了解扩张青霉在采后水果中的感染特性提供了新的视角

扩张青霉会在广泛的寄主范围内导致采后病害、果实损失和棒曲霉素（PAT）积累，并造成相当大的健康风险和经济损失。本研究调查了苹果、梨、柑橘和番茄中的动态挥发性有机化合物（VOCs）、真菌生长和 PAT 积累情况。这些指标揭示了水果对扩张菌侵袭的反应特异性。PAT 的含量从 2.54 毫克/千克到 4.48 毫克/千克不等，而在柑橘中检测到的累积量最高。利用 GC-IMS 和 HS-SPME-GC/MS 共鉴定出 247 种挥发性有机化合物，酯类是含量最高的成分，其次是醇、醛和酮。OPLS-DA 分析表明，不同的感染阶段被成功分类。此外，每种果实中分别有 21、23、19 和 34 种挥发性有机化合物与 PAT 积累有显著相关性。异戊酸和 1-己醇被确定为所有果实中的潜在生物标记物。综上所述，在适宜的条件下，扩张杆菌接种会导致果实腐烂和 PAT 积累，从而导致贮藏期间挥发性有机化合物的变化和特定挥发性物质的释放。本研究为研究扩张果实蛆与寄主之间的相互作用机制，以及早期检测扩张果实蛆感染和采后水果中 PAT 积累奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.