Developing a highly efficient fluorescence strategy for thiamine detection in real samples

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2024-09-29 DOI:10.1016/j.jphotochem.2024.116064

Safieh Momeni , Hajar Jaberie

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Abstract

This research presents the first report of a simple fluorescent approach for determination of thiamine (TH, Vitamin B1) with great sensitivity and selectivity by manganese dioxide (MnO₂) nanosheets. These nanoparticles have several advantages such as activity of oxidase like and easy, low-cost preparation. They can successfully oxidize nonfluorescent TH to intense fluorescence thiochrome at physiological pH and room temperature. Under optimized conditions and in the presence of EDTA and HCl, a linear relationship was gained between fluorescence intensities and thiamine concentrations from 10 to 5000 nM. This fluorometric method demonstrated high sensitivity with a low detection limit (5 nM). In order to confirm the selectivity of the proposed system, related interfering agents were investigated with the MnO₂ nanosheets and it was verified that the fluorescence response is greatly selective to TH. This method exhibited acceptable outcomes to evaluate TH in pharmaceutical, food, urine and serum specimens. Therefore, we suggest that the current study could be appropriate for TH measurement in biological, food and drug samples.

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开发高效荧光策略，用于检测真实样品中的硫胺素

本研究首次报道了一种利用二氧化锰（MnO2）纳米片测定硫胺素（TH，维生素 B1）的简单荧光方法，该方法具有极高的灵敏度和选择性。这些纳米颗粒具有类似氧化酶的活性和制备简单、成本低廉等优点。在生理 pH 值和室温条件下，它们能成功地将非荧光 TH 氧化成强荧光硫代铬。在乙二胺四乙酸（EDTA）和盐酸存在的优化条件下，荧光强度与硫胺浓度（10 至 5000 nM）之间呈线性关系。这种荧光测定法灵敏度高，检测限低（5 nM）。为了证实所提议系统的选择性，对相关干扰物与 MnO2 纳米片进行了研究，结果表明荧光反应对硫胺素有很大的选择性。该方法可用于评估药物、食品、尿液和血清样本中的 TH。因此，我们认为目前的研究可用于生物、食品和药物样本中 TH 的测量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.