Photodynamic surfaces coated with porphyrin-derived polymers to eradicate Staphylococcus aureus biofilms

IF 4.1 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2024-10-10 DOI:10.1016/j.jphotochem.2024.116083

María B. Ballatore , Javier E. Durantini , Claudia Solis , Milena B. Boarini , Miguel Gervaldo , Luis Otero , María E. Milanesio , Edgardo N. Durantini

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Abstract

Zn(II) porphyrin derivatives (ZnACP and ZnTCP-C₆₀) were used to obtain electrogenerated polymeric films on optically transparent indium tin oxide (ITO) electrodes. This approach produced stable and reproducible polymers that increase the lipophilicity of the surfaces. The spectroscopic and photodynamic properties of these polymeric films were compared with those corresponding to their porphyrin units. These materials photosensitized singlet molecular oxygen in water. Photoinactivation activity of the films were investigated in Staphylococcus aureus biofilms depositing on the surfaces. Thus, the ZnTCP-C₆₀ film was effective in reducing bacterial survival, reaching a photokilling of 99.7 %. In addition, ZnACP-coated surface with an absorption of 0.7 at Soret band produced a notable decrease of 4 log units in cell survival (equivalent to 99.99 % reduction) upon an exposure to white light for 60 min. Confocal scanning microscopy and scanning electron microscopy were employed to detect morphological alterations. In addition to its bactericidal effects within the biofilm, PDI using ZnACP films induced cell detachment, thereby disrupting the overall architecture of the biofilm. Thus, these polymeric films emerge as compelling photodynamic active surfaces for eradicating S. aureus bacterial biofilms.

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涂有卟啉衍生聚合物的光动力表面可消除金黄色葡萄球菌生物膜

利用锌（II）卟啉衍生物（ZnACP 和 ZnTCP-C60）在光学透明的铟锡氧化物（ITO）电极上获得电生聚合物薄膜。这种方法制备出了稳定且可重复的聚合物，增加了表面的亲油性。这些聚合物薄膜的光谱和光动力特性与其卟啉单元的相应特性进行了比较。这些材料能光敏水中的单线态分子氧。研究人员对沉积在薄膜表面的金黄色葡萄球菌生物膜进行了光灭活活性研究。结果表明，ZnTCP-C60 薄膜能有效降低细菌存活率，其光杀灭率高达 99.7%。此外，ZnACP 涂层表面在索雷特波段的吸收率为 0.7，在白光照射 60 分钟后，细胞存活率明显降低了 4 个对数单位（相当于减少 99.99%）。共焦扫描显微镜和扫描电子显微镜用于检测形态变化。除了在生物膜内的杀菌作用外，使用 ZnACP 薄膜的 PDI 还能诱导细胞脱落，从而破坏生物膜的整体结构。因此，这些聚合物薄膜是消除金黄色葡萄球菌生物膜的理想光动力活性表面。

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.