Exploring the tunable micro-/macro-structure enabled by alginate-gelatin bioinks for tissue engineering

Lucas Lemarié , Jérôme Sohier , Edwin-Joffrey Courtial
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Abstract

This study explores the development of optimized alginate-gelatin (AG) bioinks for advanced 3D bioprinting applications, particularly in tissue engineering. Central to our investigation is the establishment of a method for producing AG bioinks with highly tunable viscoelastic properties and the ability to create both macro- and micro-porous scaffolds through a liquid-liquid emulsion technique applied to chemically crosslinked hydrogels and shaped by microextrusion. Our methodology encompasses a comprehensive evaluation of homogenization, pasteurization techniques, and rheological assessments to optimize the mechanical properties of AG hydrogels, ensuring their suitability for bioprinting.
The study demonstrates that dynamic homogenization and conventional pasteurization methods yield superior dissolution and sterility of the bioinks, crucial for maintaining optical quality and biological compatibility. Crosslinking optimization significantly enhanced the elasticity and reduced post-crosslinking shrinkage of the hydrogels, a key factor in achieving desired cell viability and function within the engineered tissues. The incorporation of porosity through a controlled liquid-liquid emulsion process was found to enhance cellular interactions and integration within the bioprinted constructs.
Our findings confirm that the rheological properties of bioinks play a crucial role in determining bioprintability, with temperature modulation emerging as a key tool for tailoring these characteristics. The biocompatibility and functional performance of the AG hydrogels were validated through in vitro experiments, demonstrating promising cell viability and proliferation. This research lays the groundwork for the development of advanced bioinks capable of supporting complex tissue architectures in regenerative medicine and tissue engineering. By marrying the versatility of alginate and gelatin with innovative fabrication techniques, our study advances the frontier of 3D bioprinting, paving the way for the creation of biomimetic tissues with enhanced physiological relevance and therapeutic potential.
探索用于组织工程的藻酸盐-明胶生物墨水所带来的可调微/宏观结构
本研究探索开发优化的藻酸盐-明胶(AG)生物墨水,用于先进的三维生物打印应用,特别是组织工程。我们研究的核心是建立一种生产 AG 生物墨水的方法,这种生物墨水具有高度可调的粘弹性能,并能通过应用于化学交联水凝胶的液-液乳化技术和微挤压成型技术创建大孔和微孔支架。我们的方法包括对均质化、巴氏杀菌技术和流变学评估的全面评估,以优化 AG 水凝胶的机械性能,确保其适用于生物打印。研究表明,动态均质化和传统巴氏杀菌方法可使生物沉淀物获得优异的溶解性和无菌性,这对保持光学质量和生物兼容性至关重要。交联优化大大增强了水凝胶的弹性,减少了交联后的收缩,这是在工程组织中实现所需的细胞活力和功能的关键因素。我们的研究结果证实,生物水凝胶的流变特性在决定生物打印性方面起着至关重要的作用,而温度调节则是定制这些特性的关键工具。AG 水凝胶的生物相容性和功能性能通过体外实验得到了验证,显示出良好的细胞活力和增殖能力。这项研究为开发能够支持再生医学和组织工程中复杂组织结构的先进生物墨水奠定了基础。通过将海藻酸盐和明胶的多功能性与创新制造技术相结合,我们的研究推动了三维生物打印技术的发展,为创造具有更强生理相关性和治疗潜力的仿生组织铺平了道路。
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来源期刊
Biomedical engineering advances
Biomedical engineering advances Bioengineering, Biomedical Engineering
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