The effects of local delivery of laurus nobilis extract and adipose derived stem cells via electrospun gelatin scaffold on spinal cord injury inflammatoradscy response and its regeneration

IF 3.4 3区环境科学与生态学 Q3 CELL & TISSUE ENGINEERING

Regenerative Therapy Pub Date : 2024-06-01 DOI:10.1016/j.reth.2024.09.008

Chong Zhang , Jin Zhang , Daotao Xie , Gang Guo , Saman Jalili

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引用次数: 0

Abstract

When subjected to injury, the spinal cord's inherent complexity poses significant challenges for effective healing. In this study, gelatin nanofibers loaded with Laurus nobilis extract were developed to serve as a delivery system for adipose-derived stem cells (ADSCs), aiming to explore its potential immunomodulatory effects in a rat model of spinal cord injury. Through a series of in vitro assessments including scanning electron microscopy imaging, cell viability, anti-inflammatory, cell adhesion, biodegradation, and hemocompatibility assays, the characteristics of the delivery system were thoroughly evaluated. The in vitro studies revealed both the biocompatibility of the scaffolds and their notable anti-inflammatory properties, laying the groundwork for further investigation. Subsequent in vivo experiments demonstrated that rats treated with Laurus nobilis extract and ADSCs loaded scaffolds exhibited heightened functional recovery (BBB score of 14.66 ± 1.52 % and hot plate latency time of 8.33 0.26 s) and histological restoration at the 8-week mark post-injury. Notably, ELISA assay results revealed a significant reduction in tissue expression levels of key pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6, suggesting a pronounced immunomodulatory effect of the Laurus nobilis extract-loaded scaffolds. The findings underscore the potential of this novel delivery system to improve clinical outcomes in spinal cord injury by enhancing functional recovery and reducing inflammation. This approach could lead to the development of new, natural-based therapeutic strategies for spinal cord injury, with potential extensions to other inflammatory or degenerative conditions. Future research should focus on optimizing this strategy in larger animal models and eventually translating these findings into human clinical trials.

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通过电纺明胶支架局部输送月桂提取物和脂肪干细胞对脊髓损伤炎症细胞反应及其再生的影响

当脊髓受到损伤时，其固有的复杂性给有效愈合带来了巨大挑战。本研究开发了负载月桂萃取物的明胶纳米纤维，作为脂肪源性干细胞（ADSCs）的输送系统，旨在探索其在脊髓损伤大鼠模型中的潜在免疫调节作用。通过一系列体外评估，包括扫描电子显微镜成像、细胞存活率、抗炎、细胞粘附、生物降解和血液相容性检测，对该递送系统的特性进行了全面评估。体外研究揭示了支架的生物相容性及其显著的抗炎特性，为进一步研究奠定了基础。随后的体内实验表明，用月桂萃取物和ADSCs负载支架治疗的大鼠在损伤后8周表现出更强的功能恢复能力（BBB评分为14.66 ± 1.52%，热板潜伏时间为8.33 0.26秒）和组织学恢复能力。值得注意的是，ELISA 检测结果显示，组织中主要促炎细胞因子（包括 TNF-α、IL-1β 和 IL-6）的表达水平显著降低，这表明负载月桂萃取物的支架具有明显的免疫调节作用。这些发现强调了这种新型输送系统通过增强功能恢复和减少炎症来改善脊髓损伤临床疗效的潜力。这种方法可以为脊髓损伤开发新的天然治疗策略，并有可能扩展到其他炎症或退行性疾病。未来的研究应侧重于在更大的动物模型中优化这种策略，并最终将这些发现转化为人体临床试验。

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来源期刊

Regenerative Therapy Engineering-Biomedical Engineering

CiteScore

6.00

自引率

2.30%

发文量

106

审稿时长

49 days

期刊介绍： Regenerative Therapy is the official peer-reviewed online journal of the Japanese Society for Regenerative Medicine. Regenerative Therapy is a multidisciplinary journal that publishes original articles and reviews of basic research, clinical translation, industrial development, and regulatory issues focusing on stem cell biology, tissue engineering, and regenerative medicine.