Hydrogen Sulfide Caused a Modification in the Structure of Mitochondrial Membranes of Epicotyls of Pea Pisum sativum L. Seedlings under Water Stress

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2024-09-27 DOI:10.1134/S0006350924700283

N. Yu. Gerasimov, O. V. Nevrova, I. V. Zhigacheva, I. P. Generozova, A. N. Goloshchapov

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Abstract

The change in the structural characteristics of mitochondrial membranes isolated from epicotyls of Nemchinovsky 100 pea seedlings under the effect of sodium hydrosulfide at various concentrations has been studied. It was shown that seed treatment with sodium hydrosulfide solution at doses of 2 × 10^–4 M and 5 × 10^–6 M caused a shift of thermally induced structural transitions in mitochondrial membranes to lower temperatures and to an increase in the microviscosity of both lipid and protein regions of mitochondrial membranes with a decrease in their crystallinity. The presence of exogenous NaHS, as well as endogenous hydrogen sulfide, could increase the activity of antioxidant defense enzymes and caused the accumulation of osmolytes. As a result, the microviscosity of the lipid bilayer could increase. To test this hypothesis, the effect of the antioxidant resveratrol on the microviscosity of the mitochondrial lipid bilayer of epicotyles of pea seedlings was studied with both untreated and pretreated with NaHS seeds. The bioenergetic characteristics of mitochondria were also studied. Data have been obtained that confirm the effect of hydrogen sulfide on the structural characteristics of mitochondrial membranes by activating enzymes of antioxidant protection and accumulation of osmolytes.

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硫化氢导致水分胁迫下豌豆幼苗外胚叶线粒体膜结构的改变

研究人员研究了在不同浓度的硫氢化钠作用下，从 Nemchinovsky 100 豌豆幼苗的外胚叶中分离出的线粒体膜的结构特征变化。结果表明，用剂量为 2 × 10-4 M 和 5 × 10-6 M 的硫氢化钠溶液处理种子会导致线粒体膜的热诱导结构转变向低温转移，线粒体膜脂质和蛋白质区域的微粘度增加，结晶度降低。外源 NaHS 和内源硫化氢的存在可提高抗氧化防御酶的活性，并导致渗透溶质的积累。因此，脂质双分子层的微粘度可能会增加。为了验证这一假设，研究了抗氧化剂白藜芦醇对未经处理和预处理过 NaHS 种子的豌豆幼苗外胚层线粒体脂质双分子层微粘度的影响。还研究了线粒体的生物能特征。获得的数据证实，硫化氢通过激活抗氧化保护酶和积累渗透溶质，对线粒体膜的结构特征产生了影响。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.