Antioxidant Effect of Carnosine and Carnosine Dinitrosyl Iron Complexes under the Conditions Modeling Peroxidation of Biomolecules

IF 1 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
E. I. Nasybullina, O. V. Kosmachevskaya, K. B. Shumaev, A. F. Topunov
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Abstract

The antioxidant activity of carnosine and of carnosine dinitrosyl iron complexes (DNICs) was studied. A system with metmyoglobin (metMb) or hemin in combination with tert-butyl hydroperoxide (t-BOOH) was used as an experimental model. Using the luminol-dependent chemiluminescence method, it was shown that carnosine and carnosine DNICs effectively diminished the level of prooxidants formed by the interaction of heme groups with t-BOOH. In addition, carnosine and carnosine DNICs inhibited the formation of diene conjugates arising during the oxidation of arachidonic acid in the metMb–t-BOOH system. In the reaction systems used, the antioxidant effect of carnosine DNICs was higher than that of carnosine. The antioxidant effect of carnosine also depended on the presence of bivalent iron ions added at a concentration equivalent to their content in DNICs. These results show that the insertion of carnosine as a ligand to nitrosyl iron complexes enhances its antioxidant properties.

Abstract Image

肉碱和肉碱二亚硝基铁复合物在生物大分子过氧化模型条件下的抗氧化作用
研究了肌肽和肌肽二亚硝基铁复合物(DNIC)的抗氧化活性。实验模型是一个由偏肌红蛋白(metMb)或血红素与叔丁基过氧化氢(t-BOOH)组成的系统。使用发光酚依赖性化学发光法表明,肌肽和肌肽二硝基氯化酶能有效降低血红素基团与叔丁基过氧化氢(t-BOOH)相互作用形成的原氧化剂的水平。此外,肌肽和肌肽二硝基异构体还能抑制在 metMb-t-BOOH 系统中花生四烯酸氧化过程中产生的二烯共轭物的形成。在所使用的反应体系中,肌肽二硝基氯化酶的抗氧化效果高于肌肽。肌肽的抗氧化效果还取决于添加的二价铁离子的存在,其浓度相当于二硝基肌肽中的含量。这些结果表明,将肌肽作为配体插入亚硝基铁复合物可增强其抗氧化特性。
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来源期刊
Applied Biochemistry and Microbiology
Applied Biochemistry and Microbiology 生物-生物工程与应用微生物
CiteScore
1.70
自引率
12.50%
发文量
75
审稿时长
6-12 weeks
期刊介绍: Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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