A Study of the Influence of L-Lysine and its Transporter LysP on the Characteristics of the L-Threonine Producer Strain

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2024-01-12 DOI:10.1134/S0003683823090090

A. A. Khozov, D. M. Bubnov, T. V. Vybornaya, M. D. Kudina, A. A. Stepanova, O. E. Melkina, S. V. Molev, S. S. Filippova, A. I. Netrusov, S. P. Sineoky

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Abstract

In this work, it was shown for the first time that inactivation of the L-lysine uptake transport system from the external environment leads to an increase in the level of L-threonine synthesis by the Escherichia coli strain that produces it, while the addition of lysine to the culture medium reduces the productivity of the unmodified strain, which indicates the influence of the intracellular pool of lysine on the efficiency of threonine biosynthesis. A study of the possible mechanisms of this influence showed that neither the previously known inhibition of aspartate kinase III (LysC) activity, nor the repression of the synthesis of aspartate semialdehyde dehydrogenase (Asd) and glutamate dehydrogenase (GdhA) individually are reasons for the decrease in culture productivity. The results we obtained may indicate both the presence of a previously unknown mechanism for the regulation of threonine metabolism by lysine and the fact that several enzymes in the threonine biosynthesis pathway are simultaneously affected by this amino acid. At the same time, changing the permeability of the membrane to lysine, or reducing its intracellular concentration as a result of changing the efficiency of its biosynthesis, is a promising approach for improving threonine-producing strains.

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赖氨酸及其转运体 LysP 对赖氨酸生产菌株特性的影响研究

在这项工作中，首次证明了从外部环境中失活 L-赖氨酸吸收转运系统会导致产生 L-苏氨酸的大肠杆菌菌株合成 L-苏氨酸的水平提高，而在培养基中添加赖氨酸会降低未改良菌株的生产率，这表明细胞内赖氨酸池对苏氨酸生物合成效率的影响。对这种影响的可能机制的研究表明，无论是之前已知的抑制天门冬氨酸激酶 III（LysC）活性，还是抑制天门冬氨酸半醛脱氢酶（Asd）和谷氨酸脱氢酶（GdhA）的合成，都不是导致培养生产率下降的原因。我们获得的结果可能表明，存在一种以前未知的赖氨酸调节苏氨酸代谢的机制，而且苏氨酸生物合成途径中的几种酶同时受到这种氨基酸的影响。同时，改变膜对赖氨酸的通透性，或通过改变赖氨酸的生物合成效率来降低其在细胞内的浓度，是改良苏氨酸生产菌株的一种可行方法。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.