Yanfeng Zhu, Liping Wang, Jing Ma, Ziyi Hua, Fu Chen
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引用次数: 0
Abstract
The development of heavy metal-resistant microbial species (STS) and their interactions in the reclaimed soil play a vital role in improving the stability of mining area ecosystems. However, the development of the STS in reclaimed soil (Re) and the interactions to resist heavy metal stress (HMs) for maintaining the stability of co‑occurrence networks is still unclear. Therefore, this study employed high-throughput sequencing and differential abundance testing to explore the mechanisms of STS changes and the formation of co‑occurrence network resistance to HMs in reclaimed soil succession. Our results revealed that (1) Re treatment significantly improved the physicochemical properties of the soil and reduced the levels of HMs. Compared with the control group (CK), the heavy metal content in Re treatment decreased as follows: Hg (50.76%) > Cd (46.43%) > Cu (43.91%) > As (35.89%) > Pb (33.49%) > Cr (24.39%); (2) Compared with CK, the Shannon, ACE, and Observed species indices of bacteria in Re increased significantly by 11.70%, 549.33%, and 392.00% (p < 0.05), respectively. Additionally, Re significantly altered the β-diversity of bacterial communities (p < 0.01); (3) Proteobacteria, Acidobacteria, Bacteroidetes, and Chloroflexi were identified as the STS mediated by mining reclamation, and their changes exhibited a significant positive correlation with pH, SOM, and heavy metal content; (4) Compared with CK, the changes in STS increased the number of network modules in Re. The enrichment of Chloroflexi in the first four network modules in Re showed positive effects on Cu, Cd, and Pb stress, and taxa communities in module 3 may have a certain role in resisting As and Cr stress. Our results indicate the mechanisms of STS development and co‑occurrence network resistance in the long-term reclamation process of mining areas, thereby providing valuable insights for a deeper understanding of the role of microbes in ecological restoration of mining areas.
期刊介绍：
Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments.
Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation.
Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.
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