Enhanced detection of HER2 using a TiVC MXenes/gold nanocomposite amplified analytical biosensor for precise cancer biomarker monitoring

IF 23.2 2区 材料科学 Q1 MATERIALS SCIENCE, COMPOSITES
Najmeh Zare, Hassan Karimi-Maleh, Zhouxiang Zhang, Yangpin Wen, Nianbing Zhong, Li Fu
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Abstract

Recently, aptamers have been widely used in the detection and measurement of cancer biomarkers. This issue has had a significant impact on the process of diagnosing all types of cancers. This research work explores the development and application of layer-by-layer modified electrochemical apta-sensor for the precise monitoring of HER2, a crucial biomarker associated with breast cancer. The surface of the screen-printed carbon electrode was modified with gold nanoparticle (Au-NP) and TiVC MXene catalyst plus Pb2+ loaded aptamer (SPCE/TiVC-MXene/Au NPs/Pb2+-aptamer), which showed a high selectivity and affinity towards HER2 and offered a sensitive detection platform. The MXene nano-layer was synthesized and characterized by XPS, MAP, EDS, AFM, BET, and TEM methods and used as a substrate to improve electrochemical conductivity and loading of biological recognition element. The square-wave anodic stripping voltammetry (SWASV) method was used as a highly sensitive platform in HER2 detection. The difference of stripping signals of the Pb2+ from the SPCE/TiVC-MXene/Au NPs/Pb2+-aptamer before and after incubation in HER2 solution was selected as analytical response to achieve a reliable and quantitative analysis for HER2 concentrations. The effective factors in monitoring of HER2 such as concentration of Pb2+, incubation time, and buffer type were optimized and results showed that 5 mM of Pb2+ and 90-min incubation time in Tris–HCl created best condition in fabrication of biosensor. The results demonstrate a linear dynamic range of 1.0–1200 pg/mL for monitoring of HER2 with limit of detection of 50 fg/mL. A good affinity of fabricated apta-sensor to HER2 in the presence some other biomarkers such as PR, ER, and CEA confirmed the selectivity of the fabricated biosensor towards HER2 detection.

利用 TiVC MXenes/金纳米复合材料放大分析生物传感器增强对 HER2 的检测,实现对癌症生物标记物的精确监测
最近,适配体被广泛应用于癌症生物标志物的检测和测量。这一问题对各种癌症的诊断过程产生了重大影响。这项研究工作探讨了逐层修饰电化学适配体传感器的开发和应用,以精确监测与乳腺癌相关的重要生物标志物 HER2。丝网印刷碳电极表面经金纳米粒子(Au-NP)和 TiVC MXene 催化剂以及 Pb2+ 载体(SPCE/TiVC-MXene/Au NPs/Pb2+-aptamer)修饰,对 HER2 具有高选择性和亲和性,提供了一个灵敏的检测平台。通过 XPS、MAP、EDS、AFM、BET 和 TEM 方法合成并表征了 MXene 纳米层,并将其用作基底以提高电化学传导性和生物识别元素的负载。方波阳极剥离伏安法(SWASV)被用作检测 HER2 的高灵敏平台。该方法以 SPCE/TiVC-MXene/Au NPs/Pb2+-aptamer 在 HER2 溶液中孵育前后 Pb2+ 的剥离信号差作为分析响应,以实现对 HER2 浓度的可靠定量分析。结果表明,5 mM 的 Pb2+和 90 分钟的 Tris-HCl 培养时间为生物传感器的制作创造了最佳条件。结果表明,HER2 监测的线性动态范围为 1.0-1200 pg/mL,检测限为 50 fg/mL。在存在其他生物标志物(如 PR、ER 和 CEA)的情况下,所制造的 apta 传感器对 HER2 具有良好的亲和力,这证实了所制造的生物传感器对 HER2 检测的选择性。
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来源期刊
CiteScore
26.00
自引率
21.40%
发文量
185
期刊介绍: Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field. The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest. Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials. Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
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