Carnosic acid ameliorates postinflammatory hyperpigmentation by inhibiting inflammatory reaction and melanin deposition

IF 6.9 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomedicine & Pharmacotherapy Pub Date : 2024-10-10 DOI:10.1016/j.biopha.2024.117522

Heyi Su , Fan Yang , Keyi Lu , Jiaxian Ma , Guangming Huo , Shengjie Li , Jianmei Li

{"title":"Carnosic acid ameliorates postinflammatory hyperpigmentation by inhibiting inflammatory reaction and melanin deposition","authors":"Heyi Su , Fan Yang , Keyi Lu , Jiaxian Ma , Guangming Huo , Shengjie Li , Jianmei Li","doi":"10.1016/j.biopha.2024.117522","DOIUrl":null,"url":null,"abstract":"<div><div>The potential therapeutic effects of carnosic acid (CA) on postinflammatory hyperpigmentation (PIH) were evaluated, including its effects on melanin deposition in zebrafish, melanogenesis and melanosome transfer in skin cells and skin wound healing in mice. Our results demonstrated that CA dose-dependently decreased melanin deposition in the skin of juvenile zebrafishes. It also inhibited melanogenesis in melanoma cells and melanosome transfer to keratinocytes. Next, CA was loaded in a liposome-hydrogel system (LP-GEL) to treat skin wounds in mice. The results showed that CA-LP-GEL, as well as LP-GEL, could accelerate skin wound healing and repair the structure of healing skins in mice. Comparatively, the levels of inflammatory factors (IL-1β and TNF-a) in healing skins were significantly increased by LP-GEL, but reduced by CA-LP-GEL. In addition, Fontana-Masson staining analysis of healing skin showed that the melanoma cells were restored by the treatment of LP-GEL and CA-LP-GEL, while the melanin content was significantly increased only by LP-GEL. Real-time PCR data showed that CA decreased the gene expression related with melanogenesis (MITF, TYR and TRP-1), melanosome transfer (MLPH, Myova and Rab27a) and inflammatory cytokines (IL-1β and TNF-α) <em>in vitro</em> and <em>in vivo</em>. In conclusion, CA could reduce melanin deposition in the skin by inhibiting melanogenesis and melanosome transfer. CA-LP-GEL was found to accelerate skin wound healing and suppress inflammation and hyperpigmentation in mice. These results suggest that CA has a big developing potentiality for PIH treatment.</div></div>","PeriodicalId":8966,"journal":{"name":"Biomedicine & Pharmacotherapy","volume":"180 ","pages":"Article 117522"},"PeriodicalIF":6.9000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Pharmacotherapy","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0753332224014082","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

The potential therapeutic effects of carnosic acid (CA) on postinflammatory hyperpigmentation (PIH) were evaluated, including its effects on melanin deposition in zebrafish, melanogenesis and melanosome transfer in skin cells and skin wound healing in mice. Our results demonstrated that CA dose-dependently decreased melanin deposition in the skin of juvenile zebrafishes. It also inhibited melanogenesis in melanoma cells and melanosome transfer to keratinocytes. Next, CA was loaded in a liposome-hydrogel system (LP-GEL) to treat skin wounds in mice. The results showed that CA-LP-GEL, as well as LP-GEL, could accelerate skin wound healing and repair the structure of healing skins in mice. Comparatively, the levels of inflammatory factors (IL-1β and TNF-a) in healing skins were significantly increased by LP-GEL, but reduced by CA-LP-GEL. In addition, Fontana-Masson staining analysis of healing skin showed that the melanoma cells were restored by the treatment of LP-GEL and CA-LP-GEL, while the melanin content was significantly increased only by LP-GEL. Real-time PCR data showed that CA decreased the gene expression related with melanogenesis (MITF, TYR and TRP-1), melanosome transfer (MLPH, Myova and Rab27a) and inflammatory cytokines (IL-1β and TNF-α) in vitro and in vivo. In conclusion, CA could reduce melanin deposition in the skin by inhibiting melanogenesis and melanosome transfer. CA-LP-GEL was found to accelerate skin wound healing and suppress inflammation and hyperpigmentation in mice. These results suggest that CA has a big developing potentiality for PIH treatment.

查看原文本刊更多论文

肉苁蓉酸能抑制炎症反应和黑色素沉积，从而改善炎症后色素沉着。

我们评估了肉毒碱（CA）对炎症后色素沉着（PIH）的潜在治疗作用，包括其对斑马鱼黑色素沉积、皮肤细胞黑色素生成和黑色素小体转移以及小鼠皮肤伤口愈合的影响。我们的研究结果表明，CA 可剂量依赖性地减少斑马鱼幼鱼皮肤中的黑色素沉积。它还能抑制黑色素瘤细胞的黑色素生成和黑色素小体向角质细胞的转移。接着，CA 被载入脂质体-水凝胶系统（LP-GEL），用于治疗小鼠的皮肤伤口。结果表明，CA-LP-GEL 和 LP-GEL 都能加速小鼠皮肤伤口的愈合，并修复愈合皮肤的结构。相比之下，LP-GEL能显著提高愈合皮肤中炎症因子（IL-1β和TNF-a）的水平，而CA-LP-GEL则能降低其水平。此外，对愈合皮肤的 Fontana-Masson 染色分析表明，黑色素瘤细胞在 LP-GEL 和 CA-LP-GEL 的处理下得到恢复，而黑色素含量仅在 LP-GEL 的处理下明显增加。实时 PCR 数据显示，CA 可降低黑色素生成相关基因（MITF、TYR 和 TRP-1）、黑色素小体转移相关基因（MLPH、Myova 和 Rab27a）和炎症细胞因子（IL-1β 和 TNF-α）在体外和体内的表达。总之，CA 可通过抑制黑色素生成和黑色素小体转移来减少皮肤中的黑色素沉积。研究发现，CA-LP-GEL能加速小鼠皮肤伤口愈合，抑制炎症和色素沉着。这些结果表明，CA 在治疗 PIH 方面具有很大的发展潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedicine & Pharmacotherapy 医学-药学

CiteScore

11.90

自引率

2.70%

发文量

1621

审稿时长

48 days

期刊介绍： Biomedicine & Pharmacotherapy stands as a multidisciplinary journal, presenting a spectrum of original research reports, reviews, and communications in the realms of clinical and basic medicine, as well as pharmacology. The journal spans various fields, including Cancer, Nutriceutics, Neurodegenerative, Cardiac, and Infectious Diseases.