Accelerated mitochondrial dynamics promote spermatogonial differentiation.

IF 5.9 2区 医学 Q1 CELL & TISSUE ENGINEERING
Stem Cell Reports Pub Date : 2024-11-12 Epub Date: 2024-10-10 DOI:10.1016/j.stemcr.2024.09.006
Zhaoran Zhang, Junru Miao, Hanben Wang, Izza Ali, Duong Nguyen, Wei Chen, Yuan Wang
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引用次数: 0

Abstract

At different stages of spermatogenesis, germ cell mitochondria differ remarkably in morphology, architecture, and functions. However, it remains elusive how mitochondria change their features during spermatogonial differentiation, which in turn impacts spermatogonial stem cell fate decision. In this study, we observed that mitochondrial fusion and fission were both upregulated during spermatogonial differentiation. As a result, the mitochondrial morphology remained unaltered. Enhanced mitochondrial fusion and fission promoted spermatogonial differentiation, while the deficiency in DRP1-mediated fission led to a stage-specific blockage of spermatogenesis at differentiating spermatogonia. Our data further revealed that increased expression of pro-fusion factor MFN1 upregulated mitochondrial metabolism, whereas DRP1 specifically regulated mitochondrial permeability transition pore opening in differentiating spermatogonia. Taken together, our findings unveil how proper spermatogonial differentiation is precisely controlled by concurrently accelerated and properly balanced mitochondrial fusion and fission in a germ cell stage-specific manner, thereby providing critical insights about mitochondrial contribution to stem cell fate decision.

线粒体动力学的加速促进了精原细胞的分化。
在精子发生的不同阶段,生殖细胞线粒体在形态、结构和功能上有显著差异。然而,线粒体如何在精原细胞分化过程中改变其特征,进而影响精原干细胞命运的决定,仍然是一个未知数。在这项研究中,我们观察到在精原细胞分化过程中,线粒体的融合和分裂都被上调。因此,线粒体形态保持不变。线粒体融合和裂变的增强促进了精原细胞的分化,而 DRP1 介导的裂变的缺乏则导致精原细胞分化阶段的精子发生受阻。我们的数据进一步揭示,促融合因子 MFN1 的表达增加会上调线粒体代谢,而 DRP1 则会特异性地调节分化精原细胞中线粒体通透性转换孔的开放。综上所述,我们的研究结果揭示了精原细胞的正常分化是如何通过同时加速和适当平衡线粒体融合与分裂,以生殖细胞阶段特异性的方式精确控制的,从而为线粒体对干细胞命运决定的贡献提供了重要的启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Stem Cell Reports
Stem Cell Reports CELL & TISSUE ENGINEERING-CELL BIOLOGY
CiteScore
10.50
自引率
1.70%
发文量
200
审稿时长
28 weeks
期刊介绍: Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.
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