Relative Uptake of Tomato Carotenoids by In Vitro Intestinal and Prostate Cancer Cells

IF 3.7 3区医学 Q2 NUTRITION & DIETETICS

Journal of Nutrition Pub Date : 2024-12-01 DOI:10.1016/j.tjnut.2024.10.012

Nancy E Moran , Brianna Alexander , Shivi Garg , Nathan Marchant , Noor A Hason

{"title":"Relative Uptake of Tomato Carotenoids by In Vitro Intestinal and Prostate Cancer Cells","authors":"Nancy E Moran , Brianna Alexander , Shivi Garg , Nathan Marchant , Noor A Hason","doi":"10.1016/j.tjnut.2024.10.012","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Consumption of tomatoes and tomato carotenoids is associated with a reduced risk of prostate cancer. Prostate tissue accumulates tomato carotenoids, including lycopene, β-carotene, and phytoene. Phytoene accumulation is relatively greater in the prostate than that of lycopene, but the metabolic determinants of tissue carotenoid profiles are poorly understood.</div></div><div><h3>Objectives</h3><div>The purpose of this study was to determine if differences in stability, cellular uptake, and clearance of phytoene compared with lycopene or β-carotene by prostate and intestinal cells may explain differences in observed tissue carotenoid profiles.</div></div><div><h3>Methods</h3><div>Gene and protein expression for carotenoid metabolism in prostate cell lines were analyzed by qRT-PCR and Western blot, respectively. Uptake, efflux, and clearance of phytoene, lycopene, or β-carotene by prostate cell [LNCaP (Lymph Node Carcinoma of the Prostate cell line), RWPE-1 (a human prostate epithelial cell line), and PC-3 (aprostate cancer cell line)] and absorptive enterocyte (Caco-2) cultures were compared. The effect of scavenger receptor class B member 1 (SCARB1) inhibition on carotenoid uptake by LNCaP, RWPE-1, and Caco-2 cells was tested.</div></div><div><h3>Results</h3><div>SCARB1 was expressed across prostate cell lines. Lycopene, phytoene, and β-carotene uptakes were similar in LNCaP and PC-3 cells, whereas RWPE-1 cells absorbed a smaller portion of the phytoene dose than lycopene or β-carotene doses. The clearance rates of carotenoids from LNCaP cells did not differ. Intestinal cell uptake of phytoene was greatest, followed by β-carotene and lycopene. SCARBI inhibitor treatment did not significantly reduce the uptake or efflux of carotenoids by LNCaP or Caco-2 cells at the dose concentration provided.</div></div><div><h3>Conclusions</h3><div>Overall, this study suggests that greater bioavailability at the point of the intestine and greater stability of phytoene are determinants of the relative enrichment of phytoene in prostate tissue.</div></div>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":"154 12","pages":"Pages 3639-3651"},"PeriodicalIF":3.7000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutrition","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022316624010794","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Consumption of tomatoes and tomato carotenoids is associated with a reduced risk of prostate cancer. Prostate tissue accumulates tomato carotenoids, including lycopene, β-carotene, and phytoene. Phytoene accumulation is relatively greater in the prostate than that of lycopene, but the metabolic determinants of tissue carotenoid profiles are poorly understood.

Objectives

The purpose of this study was to determine if differences in stability, cellular uptake, and clearance of phytoene compared with lycopene or β-carotene by prostate and intestinal cells may explain differences in observed tissue carotenoid profiles.

Methods

Gene and protein expression for carotenoid metabolism in prostate cell lines were analyzed by qRT-PCR and Western blot, respectively. Uptake, efflux, and clearance of phytoene, lycopene, or β-carotene by prostate cell [LNCaP (Lymph Node Carcinoma of the Prostate cell line), RWPE-1 (a human prostate epithelial cell line), and PC-3 (aprostate cancer cell line)] and absorptive enterocyte (Caco-2) cultures were compared. The effect of scavenger receptor class B member 1 (SCARB1) inhibition on carotenoid uptake by LNCaP, RWPE-1, and Caco-2 cells was tested.

Results

SCARB1 was expressed across prostate cell lines. Lycopene, phytoene, and β-carotene uptakes were similar in LNCaP and PC-3 cells, whereas RWPE-1 cells absorbed a smaller portion of the phytoene dose than lycopene or β-carotene doses. The clearance rates of carotenoids from LNCaP cells did not differ. Intestinal cell uptake of phytoene was greatest, followed by β-carotene and lycopene. SCARBI inhibitor treatment did not significantly reduce the uptake or efflux of carotenoids by LNCaP or Caco-2 cells at the dose concentration provided.

Conclusions

Overall, this study suggests that greater bioavailability at the point of the intestine and greater stability of phytoene are determinants of the relative enrichment of phytoene in prostate tissue.

查看原文本刊更多论文

体外肠癌细胞和前列腺癌细胞对西红柿类胡萝卜素的相对吸收。

背景：食用番茄和番茄类胡萝卜素与降低前列腺癌风险有关。前列腺组织会积聚番茄类胡萝卜素，包括番茄红素、β-胡萝卜素和植物红素。与番茄红素相比，植二烯在前列腺中的积累相对较多，但人们对组织类胡萝卜素的代谢决定因素知之甚少：本研究的目的是确定前列腺细胞和肠道细胞对植物胡萝卜素与番茄红素或β-胡萝卜素的稳定性、细胞摄取量和清除率的差异是否可以解释所观察到的组织类胡萝卜素特征的差异：方法：分别通过 qRT-PCR 和 Western 印迹分析前列腺细胞系类胡萝卜素代谢的基因和蛋白质表达。比较了前列腺细胞（LNCaP、RWPE-1 和 PC-3）和吸收性肠细胞（Caco-2）对植物烯、番茄红素或 β-胡萝卜素的吸收、外流和清除。测试了清道夫受体 B 类成员 1（SCARB1）抑制对 LNCaP、RWPE-1 和 Caco-2 细胞摄取类胡萝卜素的影响：结果：SCARB1 在所有前列腺细胞系中均有表达。LNCaP和PC-3细胞对番茄红素、植物红素和β-胡萝卜素的吸收相似，而RWPE-1细胞对植物红素剂量的吸收率低于番茄红素或β-胡萝卜素剂量。类胡萝卜素在 LNCaP 细胞中的清除率没有差异。细胞肠道对植物红素的吸收率最高，其次是β-胡萝卜素和番茄红素。在所提供的剂量水平上，SR-BI 抑制剂处理并未显著减少 LNCaP 或 Caco-2 细胞对类胡萝卜素的吸收或外流：总之，这项研究表明，植物胡萝卜素在肠道中更高的生物利用度和更高的稳定性是植物胡萝卜素在前列腺组织中相对富集的决定因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Nutrition 医学-营养学

CiteScore

7.60

自引率

4.80%

发文量

260

审稿时长

39 days

期刊介绍： The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.