Phenotypic, transcriptomic, and spatial characterization of CD45RB+ naïve mature B cells: Implications in Sjögren's disease

IF 4.5 3区医学 Q2 IMMUNOLOGY

Clinical immunology Pub Date : 2024-10-10 DOI:10.1016/j.clim.2024.110378

Marina Boudigou , Marie Frutoso , Patrice Hémon , Christelle Le Dantec , Loukas Chatzis , Valérie Devauchelle , Christophe Jamin , Divi Cornec , Jacques-Olivier Pers , Laëtitia Le Pottier , Sophie Hillion

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Abstract

The conventional classification of mature B cells overlooks the diversity within IgD⁺ CD27⁻ naïve B cells. Here, to identify distinct mature naïve B cells, we categorized CD45RB^MEM55- B cells (NA RB-) and CD45RB^MEM55+ B cells (NA RB+) and explore their function and localization in circulation and tissues under physiological and pathological conditions. NA RB+ B cells, found in secondary lymphoid organs, differentiate into plasmablasts and secrete IgM. In Sjögren's disease, their numbers decrease, and they show over-activation and abnormal migration, suggesting an adaptive disease response. NA RB+ B cells also appear in inflamed salivary glands, indicating involvement in local immune responses. These findings highlight the distinct roles of NA RB+ B cells in health and Sjögren's disease.

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CD45RB+幼稚成熟B细胞的表型、转录组和空间特征：对斯约格伦病的影响。

成熟 B 细胞的传统分类忽略了 IgD+ CD27- 幼稚 B 细胞的多样性。在这里，为了识别不同的成熟幼稚B细胞，我们将CD45RBMEM55- B细胞（NA RB-）和CD45RBMEM55+ B细胞（NA RB+）进行了分类，并探讨了它们在生理和病理条件下在血液循环和组织中的功能和定位。NA RB+ B 细胞存在于继发性淋巴器官中，可分化为浆细胞并分泌 IgM。在斯约格伦病中，它们的数量会减少，并表现出过度激活和异常迁移，这表明这是一种适应性疾病反应。NA RB+ B 细胞也出现在发炎的唾液腺中，表明它们参与了局部免疫反应。这些发现凸显了NA RB+ B细胞在健康和斯约格伦病中的不同作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical immunology 医学-免疫学

CiteScore

12.30

自引率

1.20%

发文量

212

审稿时长

34 days

期刊介绍： Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.