Interactions between Helcococcus kunzii and Staphylococcus aureus: How a commensal bacterium modulates the virulence and metabolism of a pathogen in a chronic wound in vitro model.
Benjamin A R N Durand, Riham Daher, Lucia Grenga, Madjid Morsli, Jean Armengaud, Jean-Philippe Lavigne, Catherine Dunyach-Remy
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引用次数: 0
Abstract
Background: Staphylococcus aureus is the predominant pathogen isolated in diabetic foot infections. Recently, the skin commensal bacterium, Helcococcus kunzii, was found to modulate the virulence of this pathogen in an in vivo model. This study aims to elucidate the molecular mechanisms underlying the interaction between these two bacterial species, using a proteomic approach.
Results: Our results reveal that H. kunzii can coexist and proliferate alongside S. aureus in a Chronic Wound Media (CWM), thereby mimicking an in vitro chronic wound environment. We noted that the secreted proteome of H. kunzii induced a transcriptional effect on S. aureus virulence, resulting in a decrease in the expression level of agrA, a gene involved in quorum sensing. The observed effect could be ascribed to specific proteins secreted by H. kunzii including polysaccharide deacetylase, peptidoglycan DD-metalloendopeptidase, glyceraldehyde-3-phosphate dehydrogenase, trypsin-like peptidase, and an extracellular solute-binding protein. These proteins potentially interact with the agr system, influencing S. aureus virulence. Additionally, the virulence of S. aureus was notably affected by modifications in iron-related pathways and components of cell wall architecture in the presence of H. kunzii. Furthermore, the overall metabolism of S. aureus was reduced when cocultured with H. kunzii.
Conclusion: Future research will focus on elucidating the role of these excreted factors in modulating virulence.
背景:金黄色葡萄球菌是糖尿病足感染中分离出的主要病原体。最近,在一个体内模型中发现皮肤共生细菌--昆氏螺旋球菌能调节这种病原体的毒力。本研究旨在利用蛋白质组学方法阐明这两种细菌相互作用的分子机制:我们的研究结果表明,在慢性伤口培养基(CWM)中,H. kunzii可与金黄色葡萄球菌共存和增殖,从而模拟体外慢性伤口环境。我们注意到,H. kunzii分泌的蛋白质组诱导了金黄色葡萄球菌毒力的转录效应,导致参与法定人数感应的基因agrA的表达水平下降。观察到的效应可归因于 H. kunzii 分泌的特定蛋白质,包括多糖脱乙酰化酶、肽聚糖 DD-金属内肽酶、甘油醛-3-磷酸脱氢酶、胰蛋白酶样肽酶和一种细胞外溶质结合蛋白。这些蛋白质可能与 agr 系统相互作用,影响金黄色葡萄球菌的毒力。此外,在有 H. kunzii 存在的情况下,金黄色葡萄球菌的毒力明显受到铁相关途径和细胞壁结构成分变化的影响。此外,当与 H. kunzii 共同培养时,金黄色葡萄球菌的整体代谢也会降低:今后的研究将侧重于阐明这些排泄因子在调节毒力方面的作用。
期刊介绍:
BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.