Transcriptome-wide N6-methyladenosine modification profiling of long non-coding RNAs in patients with recurrent implantation failure.

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY
Ting Wang, Lili Zhang, Wenxin Gao, Yidan Liu, Feng Yue, Xiaoling Ma, Lin Liu
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引用次数: 0

Abstract

N6-methyladenosine (m6A) is involved in most biological processes and actively participates in the regulation of reproduction. According to recent research, long non-coding RNAs (lncRNAs) and their m6A modifications are involved in reproductive diseases. In the present study, using m6A-modified RNA immunoprecipitation sequencing (m6A-seq), we established the m6A methylation transcription profiles in patients with recurrent implantation failure (RIF) for the first time. There were 1443 significantly upregulated m6A peaks and 425 significantly downregulated m6A peaks in RIF. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that genes associated with differentially methylated lncRNAs are involved in the p53 signalling pathway and amino acid metabolism. The competing endogenous RNA network revealed a regulatory relationship between lncRNAs, microRNAs and messenger RNAs. We verified the m6A methylation abundances of lncRNAs by using m6A-RNA immunoprecipitation (MeRIP)-real-time polymerase chain reaction. This study lays a foundation for further exploration of the potential role of m6A modification in the pathogenesis of RIF.

复发性植入失败患者长非编码 RNA 的全转录组 N6-甲基腺苷修饰图谱。
N6-甲基腺苷(m6A)参与了大多数生物过程,并积极参与生殖调节。最新研究发现,长非编码 RNA(lncRNA)及其 m6A 修饰参与了生殖疾病的发生。在本研究中,我们利用 m6A 修饰的 RNA 免疫沉淀测序(m6A-seq)技术,首次建立了复发性种植失败(RIF)患者的 m6A 甲基化转录谱。在 RIF 中,有 1443 个明显上调的 m6A 峰和 425 个明显下调的 m6A 峰。基因本体论和京都基因组百科全书的通路分析表明,与不同甲基化lncRNA相关的基因参与了p53信号通路和氨基酸代谢。竞争性内源性RNA网络揭示了lncRNA、microRNA和信使RNA之间的调控关系。我们利用m6A-RNA免疫沉淀(MeRIP)-实时聚合酶链反应验证了lncRNA的m6A甲基化丰度。这项研究为进一步探索 m6A 修饰在 RIF 发病机制中的潜在作用奠定了基础。
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来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
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