Prevalence and genetic diversity of Giardia duodenalis in sheep from large-scale farms in Southern Xinjiang, China

IF 2.1 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2024-10-10 DOI:10.1016/j.actatropica.2024.107428

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Abstract

Giardia duodenalis is a significant zoonotic parasite. In this study, 767 fresh fecal samples were collected randomly from six large-scale sheep farms in Southern Xinjiang, China. Initially, G. duodenalis was screened using PCR at the SSU rRNA gene. Positive samples then underwent PCR amplification at the bg, gdh, and tpi genes. The prevalence of G. duodenalis in sheep was 17.5% (134/767), with the highest prevalence observed in the 3–6 months age group at 26.8% (56/209) and the lowest in the over 12 months age group at 6.8% (14/205). Among the 134 positive samples, only Assemblage E was identified. A total of 106, 92, and 98 sequences of G. duodenalis were obtained at the gdh, tpi, and bg genes, respectively. Fourteen isolates of G. duodenalis were successfully amplified at all three genes, resulting in nine G. duodenalis multilocus genotypes (MLG) named MLG E1-MLG E14, indicating high genetic diversity. In conclusion, G. duodenalis infection in sheep from large-scale farms is common in Southern Xinjiang, China, showing geographical regional distributions and genetic diversity.

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中国南疆规模养殖场绵羊中杜氏贾第鞭毛虫的流行率和遗传多样性。

十二指肠贾第虫是一种重要的人畜共患寄生虫。本研究从中国南疆的 6 个大型养羊场随机采集了 767 份新鲜粪便样本。首先，通过 SSU rRNA 基因的 PCR 对十二指肠弧菌进行筛查。然后对阳性样本进行 bg、ghdh 和 tpi 基因的 PCR 扩增。绵羊的十二指肠球菌感染率为 17.5%（134/767），其中 3-6 月龄组感染率最高，为 26.8%（56/209），12 月龄以上组感染率最低，为 6.8%（14/205）。在 134 个阳性样本中，只发现了 E 组合。在 gdh、tpi 和 bg 基因上分别获得了 106、92 和 98 个 G. duodenalis 序列。在所有三个基因上都成功扩增出了 14 个 G. duodenalis 分离物，从而产生了 9 个 G. duodenalis 多焦点基因型（MLG），命名为 MLG E1-MLG E14，表明遗传多样性很高。总之，中国南疆地区规模化养殖场的绵羊普遍感染了十二指肠杆菌，并呈现出地理区域分布和遗传多样性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.