A coherent feed-forward loop in the Arabidopsis root stem cell organizer regulates auxin biosynthesis and columella stem cell maintenance

IF 15.8 1区生物学 Q1 PLANT SCIENCES

Nature Plants Pub Date : 2024-10-11 DOI:10.1038/s41477-024-01810-z

Mohan Sharma, Thomas Friedrich, Peter Oluoch, Ning Zhang, Federico Peruzzo, Vikram Jha, Limin Pi, Edwin Philip Groot, Noortje Kornet, Marie Follo, Ernst Aichinger, Christian Fleck, Thomas Laux

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Abstract

Stem cells in plant meristems are kept undifferentiated by signals from surrounding cells and provide the basis for continuous organ formation. In the stem cell organizer of the Arabidopsis thaliana root, the quiescent centre (QC), the WOX5 transcription factor, functions as a central hub in regulating columella stem cell (CSC) homoeostasis. However, the processes mediating WOX5 function are only poorly understood. Here we identify the transcription factor HAN as a central mediator of WOX5-regulated stem cell maintenance. HAN is required for mitotic quiescence of QC and CSC maintenance and is sufficient to induce ectopic stem cells. WOX5 and HAN repress transcription of the differentiation factor gene CDF4 in a coherent feed-forward loop (cFFL), one output of which is the expression of the auxin biosynthesis gene TAA1 and maintenance of auxin response maxima in the organizer. These findings and mathematical modelling provide a mechanistic framework for WOX5 function in the root stem cell niche.

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拟南芥根茎细胞组织者中的连贯前馈环调控着叶绿素的生物合成和结节干细胞的维持

植物分生组织中的干细胞在周围细胞信号的作用下保持未分化状态，为器官的连续形成奠定了基础。在拟南芥根的干细胞组织器中，静止中心（QC）的WOX5转录因子是调节结肠干细胞（CSC）平衡的中心枢纽。然而，人们对介导 WOX5 功能的过程知之甚少。在这里，我们发现转录因子HAN是WOX5调控干细胞维持的核心介导因子。HAN是QC有丝分裂静止和CSC维持所必需的，并足以诱导异位干细胞。WOX5和HAN在一个连贯的前馈环（cFFL）中抑制分化因子基因CDF4的转录，其输出之一是表达辅助素生物合成基因TAA1和维持组织器中的辅助素反应最大值。这些发现和数学建模为WOX5在根干细胞龛中的功能提供了一个机理框架。

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来源期刊

Nature Plants PLANT SCIENCES-

CiteScore

25.30

自引率

2.20%

发文量

196

期刊介绍： Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.