DNA metabarcoding reveals distinct bacterial and phytoplankton assemblages in the Agulhas Current and the adjacent coastal shelf

IF 3.8 1区 地球科学 Q1 LIMNOLOGY
Ross‐Lynne A. Gibb, Danielle K. L. Botha, Siddarthan Venkatachalam, Mfundo Bizani, Thomas G. Bornman, Rosemary A. Dorrington
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引用次数: 0

Abstract

The Agulhas Current is a globally important western boundary current that influences numerous processes (e.g., upwelling, biogeochemical fluxes and distribution of marine taxa) in the southwestern Indian Ocean. The oceanographic processes of the Agulhas Current are well understood, but precisely how they influence coastal ecosystem productivity remains to be elucidated. In the present foundational study, we characterized the bacterial (16S rRNA gene) and phytoplankton (chloroplast 16S rRNA, rbcL, and eukaryotic 18S rRNA genes) community structures of the Agulhas Current system using a metabarcoding approach. All four markers provided consistent data on the bacterial and phytoplankton communities in the Agulhas Current and coastal sites. The study revealed distinct, conserved communities and similar patterns of dominance by taxa adapted to oligotrophic conditions within the Agulhas Current, sampled 2 yr apart. By contrast, there was significant variability in taxonomic diversity and abundance of phytoplankton communities in the adjacent coastal waters that could be linked to localized upwelling. While the Agulhas Current bacterial and phytoplankton communities were diverse and represented many functional groups, the coastal sites were more diatom‐dominated and included genera typically associated with upwelling, for example, Thalassiosira spp. Based on their relative abundance profiles, phytoplankton communities were more responsive to environmental variability than bacteria and may therefore prove more useful in linking ecosystem dynamics to environmental variability in marine systems.
DNA 代谢编码揭示了阿古哈斯洋流和邻近沿岸陆架中不同的细菌和浮游植物群落
阿古哈斯洋流是全球重要的西边界洋流,影响印度洋西南部的许多过程(如上升流、生 物地球化学通量和海洋生物分类群的分布)。人们对阿古哈斯洋流的海洋学过程已经有了很好的了解,但它们是如何影响沿岸生态系统生产力的,还有待进一步阐明。在本基础研究中,我们采用元条码方法描述了阿古哈斯洋流系统的细菌(16S rRNA 基因)和浮游植物(叶绿体 16S rRNA、rbcL 和真核 18S rRNA 基因)群落结构。所有四种标记都提供了有关阿古哈斯海流和沿岸地点细菌和浮游植物群落的一致数据。研究发现,在阿古哈斯海流中,适应低营养条件的类群具有独特、保守的群落和相似的优势模式,取样时间相隔两年。相比之下,邻近沿岸水域的浮游植物群落在分类多样性和丰度方面存在很大差异,这可能与局部上升流有关。阿古哈斯海流的细菌和浮游植物群落种类繁多,代表了许多功能群,而沿岸地点则以硅藻为主,包括通常与上升流有关的属,如 Thalassiosira spp。
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来源期刊
Limnology and Oceanography
Limnology and Oceanography 地学-海洋学
CiteScore
8.80
自引率
6.70%
发文量
254
审稿时长
3 months
期刊介绍: Limnology and Oceanography (L&O; print ISSN 0024-3590, online ISSN 1939-5590) publishes original articles, including scholarly reviews, about all aspects of limnology and oceanography. The journal''s unifying theme is the understanding of aquatic systems. Submissions are judged on the originality of their data, interpretations, and ideas, and on the degree to which they can be generalized beyond the particular aquatic system examined. Laboratory and modeling studies must demonstrate relevance to field environments; typically this means that they are bolstered by substantial "real-world" data. Few purely theoretical or purely empirical papers are accepted for review.
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