Quantification of porcine lower thoracic spinal cord morphology with intact dura mater using high-resolution μCT

IF 2.3 4区医学 Q3 CLINICAL NEUROLOGY

Journal of Neuroimaging Pub Date : 2024-10-10 DOI:10.1111/jon.13239

Justin Chin, Megan L. Settell, Meagan K. Brucker-Hahn, Daniel Lust, Jichu Zhang, Aniruddha R. Upadhye, Bruce Knudsen, Ashlesha Deshmukh, Kip A. Ludwig, Igor A. Lavrov, Andrew R. Crofton, Scott F. Lempka, Mingming Zhang, Andrew J. Shoffstall

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引用次数: 0

Abstract

Background and Purpose

Spinal cord stimulation (SCS) is approved by the Food and Drug Administration for treating chronic intractable pain in the back, trunk, or limbs through stimulation of the dorsal column. Numerous studies have used swine as an analog of the human spinal cord to better understand SCS and further improve its efficacy. We performed high-resolution imaging of the porcine spinal cord with intact dura mater using micro-computed tomography (μCT) to construct detailed 3-dimensional (3D) visualizations of the spinal cord and characterize the morphology of the dorsal and ventral rootlets.

Methods

We obtained spinal cords from Yorkshire/Landrace crossbred swine (N = 7), stained samples with osmium tetroxide, and performed μCT imaging of the T12-T15 levels at isotropic voxel resolutions ranging from 3.3 to 50 μm. We measured the anatomical morphology using the 3D volumes and compared our results to measurements previously collected from swine and human spinal cords via microdissection techniques in prior literature.

Results

While the porcine thoracic-lumbar spinal cord is a popular model for SCS, we highlight multiple notable differences compared to previously published T8-T12 human measurements including rootlet counts (porcine dorsal/ventral: 12.2 ± 2.6, 26.6 ± 3.4; human dorsal/ventral: 5.3 ± 1.3, 4.4 ± 2.4), rootlet angles (porcine ventral-rostral: 161 ± 1°, ventral-caudal: 155 ± 6°, dorsal-rostral: 148 ± 9°, dorsal-caudal: 142 ± 6°; human ventral-rostral: 170 ± 3°, ventral-caudal: 22 ± 10°, dorsal-rostral: 171 ± 3°, dorsal-caudal: 15 ± 7°), and the presence and count of dorsal rootlet bundles.

Conclusions

Detailed measurements and highlighted differences between human and porcine spinal cords can inform variations in modeling and electrophysiological experiments between the two species. In contrast to other approaches for measuring the spinal cord and rootlet morphology, our method keeps the dura intact, reducing potential artifacts from dissection.

Abstract Image

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利用高分辨率μCT对猪下胸椎脊髓形态和完整硬脑膜进行量化。

背景和目的：脊髓刺激疗法（SCS）已获美国食品和药物管理局批准，用于通过刺激背柱治疗背部、躯干或四肢的慢性顽固性疼痛。许多研究将猪作为人类脊髓的类似物，以更好地了解 SCS 并进一步提高其疗效。我们使用微型计算机断层扫描（μCT）对猪脊髓和完整硬脑膜进行了高分辨率成像，以构建脊髓的详细三维（3D）可视化图像，并确定背侧和腹侧小根的形态特征：我们从约克郡/兰德良种杂交猪（N = 7）身上获取脊髓，用四氧化锇对样本进行染色，然后以 3.3 至 50 μm 的各向同性体素分辨率对 T12 至 T15 层进行μCT 成像。我们利用三维体积测量了解剖形态，并将我们的结果与之前文献中通过显微切割技术从猪和人脊髓收集的测量结果进行了比较：4）、小根角度（猪腹喙：161 ± 1°，腹尾：155 ± 6°，背喙：148 ± 9°，背尾：142 ± 6°；人腹喙：170 ± 3°，腹尾：22 ± 10°，背喙：171 ± 3°，背尾：15 ± 7°）以及背侧小根束的存在和数量：结论：对人和猪脊髓的详细测量和突出差异可为两个物种之间的建模和电生理实验提供信息。与其他测量脊髓和小根形态的方法相比，我们的方法保持了硬脊膜的完整，减少了解剖可能造成的伪影。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Neuroimaging 医学-核医学

CiteScore

4.70

自引率

0.00%

发文量

117

审稿时长

6-12 weeks

期刊介绍： Start reading the Journal of Neuroimaging to learn the latest neurological imaging techniques. The peer-reviewed research is written in a practical clinical context, giving you the information you need on: MRI CT Carotid Ultrasound and TCD SPECT PET Endovascular Surgical Neuroradiology Functional MRI Xenon CT and other new and upcoming neuroscientific modalities.The Journal of Neuroimaging addresses the full spectrum of human nervous system disease, including stroke, neoplasia, degenerating and demyelinating disease, epilepsy, tumors, lesions, infectious disease, cerebral vascular arterial diseases, toxic-metabolic disease, psychoses, dementias, heredo-familial disease, and trauma.Offering original research, review articles, case reports, neuroimaging CPCs, and evaluations of instruments and technology relevant to the nervous system, the Journal of Neuroimaging focuses on useful clinical developments and applications, tested techniques and interpretations, patient care, diagnostics, and therapeutics. Start reading today!