Genomic characteristics of human respiratory syncytial virus from children in China during 2017-2020

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2024-10-10 DOI:10.1007/s00705-024-06138-9

Fei Li, Yun Zhu, Qiuping Li, Xiaolei Guan, Hailin Zhang, Changchong Li, Meng Zhang, Lei Li, Yiliang Fu, Yali Duan, Luci Huang, Zhengde Xie, Xiangpeng Chen

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Abstract

Acute lower respiratory tract infections (ALRTIs) are a leading cause of mortality in young children worldwide due to human respiratory syncytial virus (RSV). The aim of this study was to monitor genetic variations in RSV and provide genomic data support for RSV prevention and control. A total of 105 complete RSV genome sequences were determined during 2017-2020. Phylogenetic analysis showed that all of the RSVA sequences were of genotype ON1, and all of the RSVB sequences were of genotype BA9. Notably, a phylogenetic tree based on the whole genome had more branches than a tree based on the G gene. In comparison to the RSV prototype sequences, 71.43% (50/70) of the ON1 sequences had five amino acid substitutions (T113I, V131N, N178G, H258Q, and H266L) that occurred simultaneously, and 68.57% (24/35) of the BA9 genotype sequences had 12 amino acid substitutions, four of which (A131T, T137I, T288I, and T310I) occurred simultaneously. In the F gene, there were 19 amino acid substitutions, which were mainly located in the antigenic sites Ø, II, V, and VII. Other amino acid substitutions were found in the NS1, NS2, P, SH, and L proteins. No significant evidence of recombination was found in any of the sequences. These findings provide important data that will be useful for prevention, control, and vaccine development against RSV.

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2017-2020年中国儿童人呼吸道合胞病毒的基因组特征。

急性下呼吸道感染（ALRTIs）是人类呼吸道合胞病毒（RSV）导致全球幼儿死亡的主要原因。本研究旨在监测 RSV 的基因变异，为 RSV 的预防和控制提供基因组数据支持。2017-2020年间，共测定了105个完整的RSV基因组序列。系统进化分析表明，所有的RSVA序列均为基因型ON1，所有的RSVB序列均为基因型BA9。值得注意的是，基于全基因组的系统发生树比基于 G 基因的系统发生树有更多的分支。与 RSV 原型序列相比，71.43%（50/70）的 ON1 序列有 5 个同时发生的氨基酸置换（T113I、V131N、N178G、H258Q 和 H266L），68.57%（24/35）的 BA9 基因型序列有 12 个氨基酸置换，其中 4 个（A131T、T137I、T288I 和 T310I）同时发生。在 F 基因中，有 19 个氨基酸置换，主要位于抗原位点 Ø、II、V 和 VII。其他氨基酸替换出现在 NS1、NS2、P、SH 和 L 蛋白中。在任何序列中都没有发现重组的重要证据。这些发现为 RSV 的预防、控制和疫苗开发提供了重要数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.