Acute Respiratory Syndrome Mimicking Shipping Sickness in Theileria buffeli Infected Buffalo Calf

Abstract

Benign theileriosis, caused by the members of the Theileria orientalis complex, can develop fatal clinical outbreaks characterized by acute respiratory manifestation in stressful conditions. This report describes the molecular diagnosis and clinical management of a recently transported buffalo calf with severe Theileria buffeli infection and associated acute pneumonia. A five-month-old male buffalo calf having an inter-state travel history three days back was presented with pyrexia, anorexia, weakness, mucoid rhinorrhoea, dyspnoea and diarrhoea from the day of procurement. The history and physical examination revealed a clinical presentation similar to shipping fever. Whereas, severe parasitemia of Theileria spp. with anaemia, thrombocytopenia and granulopenia were evident on laboratory investigation. The Theileria spp. infection was confirmed by PCR method using specific primers and the authentication was made by detailed sequence analysis. The small subunit rRNA was amplified using universal apicomplexan primers and the phylogenetic analysis was carried out for further characterisation. The animal was stabilized by steroid nebulization therapy and the specific chemotherapy was instigated using buparvaquone and sulphamethoxazole-trimethoprim combination. Supportive medications like non-steroidal anti-inflammatory drugs, antihistamines, antidiarrhoeals and vitamins were provided symptomatically. The animal showed a good response to therapy and recovered from parasitemia by day 10 and the molecular clearance was later confirmed on day 70 of therapy. The present case of Theileria buffeli infected buffalo calf with acute respiratory signs points towards the possible hemoparasitic outbreaks in transport-stressed animals with the signs of shipping fever-associated syndrome.