Dietary zinc status is associated with ZnT3 (SLC30A3), IL-6 gene expressions and spinal cord tissue damage in spinal cord tissue in a cuprizone-induced rat Multiple Sclerosis model

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Aysenur Feyza Bayiroglu , Gozde Acar , Elif Gulbahce-Mutlu , Saltuk Bugra Baltaci , Rasim Mogulkoc , Abdulkerim Kasim Baltaci
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Abstract

The aim of this study was to investigate the effects of dietary zinc status on spinal cord tissue damage and ZnT3, IL-6 gene expressions in a cuprizone-induced rat Multiple Sclerosis (MS) model. The study was carried out on 46 adult male rats of the genus Wistar. The animals used in the study were divided into 5 groups (G) (Control 6, other groups 10). G1, Control. G2, Sham-MS: Carboxy-methyl-cellulose (KMS) solution in which Cuprizon was dissolved was given to rats by gavage daily for 8 weeks at the rate of 1 % of daily feed consumption. MS was formed by giving 1 % of the daily feed consumption cuprizon in KMS solution by gavage to the animals in G3, 4 and 5 for 8 weeks. G4 was fed with a zinc deficient (50 µg/kg zinc) diet. G5 was given intraperitoneal (ip) zinc sulfate (5 mg/kg/day) supplementation. MS formation in animals was determined by Rotarod tests and Myelin Basic Protein (MBP) gene expression analysis. ZNT3 and IL-6 gene expression levels in spinal cord tissue samples of animals by Real-Time-PCR method; MDA and GSH levels were determined by ELISA method. The highest spinal cord MDA and IL-6 levels were obtained in G3 and G4 (P<0.05). Zinc supplementation in G5 prevented the increase in the mentioned parameters and turned them into control values (P<0.05). The spinal cord GSH and ZnT3 levels of G3 and G4 were lower than all other groups (P<0.05). Zinc supplementation prevented suppression in the same parameters in G5 and reached the control values (P<0.05). The findings of the current study suggest that zinc supplementation in addition to treatment for MS may be beneficial in reducing the severity of the disease.
在铜绿素诱导的大鼠多发性硬化症模型中,膳食锌状况与脊髓组织中 ZnT3 (SLC30A3)、IL-6 基因表达和脊髓组织损伤有关。
本研究旨在探讨饮食中的锌对铜绿素诱导的大鼠多发性硬化症(MS)模型中脊髓组织损伤和 ZnT3、IL-6 基因表达的影响。研究以 46 只成年雄性 Wistar 大鼠为对象。研究中使用的动物分为 5 组(G)(对照组 6 只,其他组 10 只)。G1,对照组。G2,Sham-MS:将 Cuprizon 溶解在羧甲基纤维素(KMS)溶液中,每天给大鼠灌胃,持续 8 周,剂量为每日饲料消耗量的 1%。给 G3、4 和 5 组的动物灌胃 KMS 溶液中 Cuprizon 的浓度为日饲料消耗量的 1%,持续 8 周,从而形成 MS。G4 用缺锌(50 微克/千克锌)的饲料喂养。G5 组动物腹腔注射硫酸锌(5 毫克/千克/天)。通过旋转测试和髓鞘碱性蛋白(MBP)基因表达分析确定动物体内多发性硬化症的形成。动物脊髓组织样本中的ZNT3和IL-6基因表达水平采用Real-Time-PCR方法测定;MDA和GSH水平采用ELISA方法测定。G3 和 G4 的脊髓 MDA 和 IL-6 水平最高(P<0.05)。
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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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