A new ex vivo model system to analyze factors affecting the integrity of fetal membranes in fetoscopic surgery

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials Pub Date : 2024-10-01 DOI:10.1016/j.jmbbm.2024.106764

Serjosha Robmann , Raoul Hopf , Costanza Giampietro , Lukas Moser , Alexandra Dolder , Magdalena Sanz Cortes , Martin Ehrbar , Nicole Ochsenbein , Jan Deprest , Edoardo Mazza

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Abstract

We developed an ex vivo model system to analyze the influence of relevant environmental and mechanical factors potentially affecting the integrity of fetal membranes during fetoscopic surgery. The set-up exposes amniochorion membranes to insufflation at predefined levels of gas pressure, flow, humidity, and temperature. Change in fetal membranes stiffness is quantified during the phase mimicking surgery through measurement of membranes’ strain in response to cyclic overpressure. The trocar induced perforation creates a mechanical weakness whose stability is assessed by increasing the insufflation pressure until membrane rupture. Damage of the epithelial cells lining the amnion is assessed through live-dead staining. Initial experiments demonstrated the functionality of the new apparatus and the feasibility of the proposed protocols. Fetal membranes exposed to air with low humidity for approximately 1 h demonstrated significant embrittlement, while their mechanical integrity was maintained in case of gas insufflation at high humidity (air as well as CO₂). Under dry circumstances, there was a significant rate of epithelial cell death. Separation of amnion and chorion in the region of the trocar site was visible in all experiments. This new model is a versatile platform for analyzing the mechanical, histological, and biological implications of fetoscopic surgery on fetal membranes.

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在胎儿镜手术中分析影响胎膜完整性因素的新型体外模型系统。

我们开发了一种体外模型系统，用于分析胎儿镜手术过程中可能影响胎膜完整性的相关环境和机械因素的影响。该装置在预定的气体压力、流量、湿度和温度水平下对羊膜进行充气。在模拟手术阶段，通过测量胎膜对循环过压的应变，量化胎膜硬度的变化。通过增加充气压力直至胎膜破裂，来评估套管穿孔造成的机械弱点的稳定性。内衬羊膜的上皮细胞受损情况通过活体死亡染色进行评估。初步实验证明了新仪器的功能性和建议方案的可行性。胎膜在低湿度空气中暴露约 1 小时后出现明显脆化，而在高湿度气体（空气和二氧化碳）充气的情况下，胎膜的机械完整性得以保持。在干燥环境下，上皮细胞的死亡率很高。在所有实验中都能看到套管部位的羊膜和绒毛分离。这种新模型是分析胎儿镜手术对胎膜的机械、组织学和生物学影响的多功能平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学：生物材料

CiteScore

7.20

自引率

7.70%

发文量

505

审稿时长

46 days

期刊介绍： The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.