Clinicopathologic Characteristics of Trop Family Proteins (Trop-2 and EpCAM) in Gastric Carcinoma.

IF 3.2 4区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY
Hye Sung Kim, Younghoon Kim, Hye Seung Lee
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Abstract

Purpose: Trop family proteins, including epithelial cell adhesion molecule (EpCAM) and Trop-2, have garnered attention as potential therapeutic and diagnostic targets for various malignancies. This study aimed to elucidate the clinicopathological significance of these proteins in gastric carcinoma (GC) and to reinforce their potential as biomarkers for patient stratification in targeted therapies.

Materials and methods: Immunohistochemical (IHC) analyses of EpCAM and Trop-2 were performed on GC and precancerous lesions, following rigorous orthogonal validation of the antibodies to ensure specificity and sensitivity.

Results: Strong membranous staining (3+) for Trop-2 was observed in 49.3% of the GC cases, whereas EpCAM was strongly expressed in almost all cases (93.2%), indicating its widespread expression in GC. A high Trop-2 expression level, characterized by an elevated H-score, was significantly associated with intestinal type by Lauren classification, gastric mucin type, presence of lymph node metastasis, human epidermal growth factor receptor 2-positivity, and Epstein-Barr virus (EBV)-positivity. Patients with a high Trop-2 expression level exhibited poorer survival outcomes on univariate and multivariate analyses. High EpCAM expression levels were prevalent in differentiated histologic type, microsatellite instability-high, and EBV-negative cancer, and were correlated with high densities of CD3 and CD8 T cells and elevated combined positive score for programmed death-ligand 1.

Conclusions: These results highlight the differential expression of Trop-2 and EpCAM and their prognostic implications in GC. The use of meticulously validated antibodies ensured the reliability of our IHC data, thereby offering a robust foundation for future therapeutic strategies targeting Trop family members in GC.

胃癌中 Trop 家族蛋白(Trop-2 和 EpCAM)的临床病理特征
目的:Trop家族蛋白,包括上皮细胞粘附分子(EpCAM)和Trop-2,作为各种恶性肿瘤的潜在治疗和诊断靶点已引起人们的关注。本研究旨在阐明这些蛋白在胃癌(GC)中的临床病理学意义,并加强它们作为生物标记物在靶向治疗中对患者进行分层的潜力:对 GC 和癌前病变进行 EpCAM 和 Trop-2 的免疫组化(IHC)分析,并对抗体进行严格的正交验证,以确保特异性和灵敏度:结果:49.3%的 GC 病例观察到 Trop-2 强膜染色(3+),而 EpCAM 几乎在所有病例(93.2%)中都有强表达,表明其在 GC 中广泛表达。以 H 评分升高为特征的 Trop-2 高表达水平与劳伦分类中的肠类型、胃粘膜类型、淋巴结转移的存在、人表皮生长因子受体 2 阳性和 Epstein-Barr 病毒(EBV)阳性显著相关。在单变量和多变量分析中,Trop-2表达水平高的患者生存率较低。EpCAM的高表达水平在分化组织学类型、微卫星不稳定性高和EBV阴性的癌症中普遍存在,并与CD3和CD8 T细胞的高密度以及程序性死亡配体1的综合阳性评分升高相关:这些结果突显了Trop-2和EpCAM的差异表达及其对GC预后的影响。使用经过严格验证的抗体确保了 IHC 数据的可靠性,从而为未来针对 Trop 家族成员的 GC 治疗策略奠定了坚实的基础。
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来源期刊
Journal of Gastric Cancer
Journal of Gastric Cancer Biochemistry, Genetics and Molecular Biology-Cancer Research
CiteScore
4.30
自引率
12.00%
发文量
36
期刊介绍: The Journal of Gastric Cancer (J Gastric Cancer) is an international peer-reviewed journal. Each issue carries high quality clinical and translational researches on gastric neoplasms. Editorial Board of J Gastric Cancer publishes original articles on pathophysiology, molecular oncology, diagnosis, treatment, and prevention of gastric cancer as well as articles on dietary control and improving the quality of life for gastric cancer patients. J Gastric Cancer includes case reports, review articles, how I do it articles, editorials, and letters to the editor.
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