A Highly Sensitive Inclusion Complex Based Spectrofluorimetric Method for the Determination of Certain Sodium Glucose Cotransporter-2 Inhibitors: Greenness Assessment and Application to Different Biological Fluids

IF 3.2 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2024-10-08 DOI:10.1002/bio.4917

Doaa H. Rushdy, Noha N. Atia, Marwa F. B. Ali, Samia M. El-Gizawy

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引用次数: 0

Abstract

This study introduces a remarkably simple, green, and highly sensitive inclusion complex based spectrofluorimetric method for analyzing two sodium glucose cotransporter-2 (SGLT2) inhibitors: empagliflozin (EGF) and dapagliflozin (DGF). The method utilizes beta-cyclodextrin (β-CD) complexation to enhance the native fluorescence of EGF and DGF in aqueous solutions, resulting in 11.0-fold and 9.0-fold intensity increases, respectively. Fluorescence measurements were conducted at 301 nm emission following 230 nm excitation for both drugs. The method demonstrates excellent linearity (0.9994 for EGF and 0.9993 for DGF) over concentration ranges of 5.0–250.0 ng/mL and 10.0–300.0 ng/mL, with low detection limits of 1.05 and 1.38 ng/mL for EGF and DGF, respectively. The method's versatility was validated through successful application in pharmaceutical formulations, content uniformity testing, and biological fluids. This eco-friendly approach primarily uses water as a solvent and requires minimal reagents. The method's environmental impact was comprehensively evaluated using the analytical eco-scale, green analytical procedure index (GAPI), and analytical greenness metric (AGREE).

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基于包合物的高灵敏度分光荧光法测定某些葡萄糖钠otransporter-2抑制剂：绿色评估及在不同生物液体中的应用。

本研究介绍了一种非常简单、绿色和高灵敏度的基于包涵复合物的分光荧光测定法，用于分析两种钠葡萄糖共转运体-2（SGLT2）抑制剂：empagliflozin (EGF)和dapagliflozin (DGF)。该方法利用β-环糊精（β-CD）复合物来增强水溶液中EGF和DGF的原生荧光，使其强度分别增加11.0倍和9.0倍。两种药物的荧光测量均在 301 nm 发射波长和 230 nm 激发波长之间进行。该方法在 5.0-250.0 纳克/毫升和 10.0-300.0 纳克/毫升的浓度范围内线性关系良好（EGF 为 0.9994，DGF 为 0.9993），EGF 和 DGF 的检测限分别为 1.05 和 1.38 纳克/毫升。通过在药物制剂、含量均匀性测试和生物液体中的成功应用，验证了该方法的多功能性。这种环保方法主要以水为溶剂，所需试剂极少。利用分析生态尺度、绿色分析程序指数（GAPI）和分析绿色度量（AGREE）对该方法的环境影响进行了全面评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.