Amalgam plays a dual role in controlling the number of leg muscle progenitors and regulating their interactions with the developing Drosophila tendon.

IF 9.8 1区 生物学 Q1 Agricultural and Biological Sciences
PLoS Biology Pub Date : 2024-10-07 eCollection Date: 2024-10-01 DOI:10.1371/journal.pbio.3002842
Blandine Moucaud, Elodie Prince, Elia Ragot, Yoan Renaud, Krzysztof Jagla, Guillaume Junion, Cedric Soler
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Abstract

Formation of functional organs requires cell-cell communication between different cell lineages and failure in this communication can result in severe developmental defects. Hundreds of possible interacting pairs of proteins are known, but identifying the interacting partners that ensure a specific interaction between 2 given cell types remains challenging. Here, we use the Drosophila leg model and our cell type-specific transcriptomic data sets to uncover the molecular mediators of cell-cell communication between tendon and muscle precursors. Through the analysis of gene expression signatures of appendicular muscle and tendon precursor cells, we identify 2 candidates for early interactions between these 2 cell populations: Amalgam (Ama) encoding a secreted protein and Neurotactin (Nrt) known to encode a membrane-bound protein. Developmental expression and function analyses reveal that: (i) Ama is expressed in the leg myoblasts, whereas Nrt is expressed in adjacent tendon precursors; and (ii) in Ama and Nrt mutants, myoblast-tendon cell-cell association is lost, leading to tendon developmental defects. Furthermore, we demonstrate that Ama acts downstream of the FGFR pathway to maintain the myoblast population by promoting cell survival and proliferation in an Nrt-independent manner. Together, our data pinpoint Ama and Nrt as molecular actors ensuring early reciprocal communication between leg muscle and tendon precursors, a prerequisite for the coordinated development of the appendicular musculoskeletal system.

汞齐在控制腿部肌肉祖细胞的数量以及调节它们与发育中果蝇肌腱的相互作用方面发挥着双重作用。
功能器官的形成需要不同细胞系之间的细胞间交流,这种交流的失败会导致严重的发育缺陷。目前已知有数百对可能存在相互作用的蛋白质,但确定确保两种特定细胞类型之间发生特定相互作用的相互作用伙伴仍然具有挑战性。在这里,我们利用果蝇腿部模型和细胞类型特异性转录组数据集来揭示肌腱和肌肉前体之间细胞-细胞通讯的分子介质。通过分析附着肌和肌腱前体细胞的基因表达特征,我们确定了这两种细胞群之间早期相互作用的两个候选者:Amalgam(Ama)编码一种分泌蛋白,Neurotactin(Nrt)编码一种膜结合蛋白。发育表达和功能分析显示(i) Ama 在腿部肌母细胞中表达,而 Nrt 则在邻近的肌腱前体中表达;(ii) 在 Ama 和 Nrt 突变体中,肌母细胞-肌腱细胞-细胞间的关联丧失,导致肌腱发育缺陷。此外,我们还证明,Ama 在表皮生长因子受体通路的下游发挥作用,以一种不依赖 Nrt 的方式促进细胞存活和增殖,从而维持肌母细胞群。总之,我们的数据指出,Ama 和 Nrt 是确保腿部肌肉和肌腱前体之间早期相互交流的分子角色,而这种交流是附属肌肉骨骼系统协调发育的先决条件。
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来源期刊
PLoS Biology
PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY
CiteScore
15.40
自引率
2.00%
发文量
359
审稿时长
3-8 weeks
期刊介绍: PLOS Biology is the flagship journal of the Public Library of Science (PLOS) and focuses on publishing groundbreaking and relevant research in all areas of biological science. The journal features works at various scales, ranging from molecules to ecosystems, and also encourages interdisciplinary studies. PLOS Biology publishes articles that demonstrate exceptional significance, originality, and relevance, with a high standard of scientific rigor in methodology, reporting, and conclusions. The journal aims to advance science and serve the research community by transforming research communication to align with the research process. It offers evolving article types and policies that empower authors to share the complete story behind their scientific findings with a diverse global audience of researchers, educators, policymakers, patient advocacy groups, and the general public. PLOS Biology, along with other PLOS journals, is widely indexed by major services such as Crossref, Dimensions, DOAJ, Google Scholar, PubMed, PubMed Central, Scopus, and Web of Science. Additionally, PLOS Biology is indexed by various other services including AGRICOLA, Biological Abstracts, BIOSYS Previews, CABI CAB Abstracts, CABI Global Health, CAPES, CAS, CNKI, Embase, Journal Guide, MEDLINE, and Zoological Record, ensuring that the research content is easily accessible and discoverable by a wide range of audiences.
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