MpPUB9, a U-box E3 ubiquitin ligase, acts as a positive regulator by promoting the turnover of MpEXO70.1 under high salinity in Marchantia polymorpha

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-10-10 DOI:10.1111/nph.20169

Cheol Jin Lim, Hyeon Ji Seo, Haijing Yin, Na Hyun Cho, Hee Woong Yang, Tae Hyeon Park, Yun Ju Kim, Woo Taek Kim, Dong Hye Seo

引用次数: 0

Abstract

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MpPUB9是一种U-box E3泛素连接酶，在高盐度条件下，它通过促进MpEXO70.1的周转起到正向调节作用。

在陆生植物单系群中处于基部位置的多孔菌（Marchantia polymorpha），与动物的U-box（PUB）蛋白相比，显示出显著的植物U-box（PUB）蛋白扩增。我们阐明了 MpPUB9 在调节多甲藻耐盐胁迫能力中的作用。高盐度和脱水迅速诱导了MpPUB9的表达。MpPUB9 的 N 端具有完整的 U-box 结构域。MpPUB9-Citrine 定位于点状结构，外周与微粒体膜相关。表型分析表明，MpPUB9的过表达和抑制所诱导的矮缩和表缩苔藓生长表型可能分别引起了抗盐胁迫和易受盐胁迫的表型。研究还发现，MpPUB9 与外囊蛋白 MpEXO70.1 直接相互作用，导致其泛素化。在高盐度条件下，虽然 MpPUB9 的稳定性显著增加，但 MpEXO70.1 的周转速度略快。转录组分析表明，盐处理和 MpPUB9 的过表达共同调控了与调节 H2O2 和细胞壁组织相关的基因。总之，我们的研究结果表明，MpPUB9在盐胁迫耐受性的正向调控中起着至关重要的作用，它与MpEXO70.1相互作用，在高盐条件下通过UPS与自噬的协调调节蛋白质的周转。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.