Proteomics analysis reveals the differential protein expression of female and male adult Toxocara canis using Orbitrap Astral analyzer.

IF 8.1 1区医学

Infectious Diseases of Poverty Pub Date : 2024-10-09 DOI:10.1186/s40249-024-01246-9

Hui-Jie Qiu, Ya-Jia Zhou, Zhi-Yu Li, Yi-Han Lv, Xing-Quan Zhu, Wen-Bin Zheng

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Abstract

Background: Toxocara canis, the most prevalent helminth in dogs and other canines, is one of the socioeconomically important zoonotic parasites, particularly affecting pediatric and adolescent populations in impoverished communities. However, limited information is available regarding the proteomes of female and male adult T. canis. To address this knowledge gap, we performed a comprehensive proteomic analysis to identify the proteins with differential abundance (PDAs) and gender-specifically expressed proteins between the two sexes adult T. canis.

Methods: The comparative proteomic analysis was carried out by the Orbitrap mass spectrometry (MS) with asymmetric track lossless (Astral) analyzer. The difference analysis was conducted using t-test and the proteins verification was achieved through parallel reaction monitoring (PRM). The potential biological functions of identified adult T. canis proteins and PDAs were predicted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The domain, transcription factor and subcellular localization of the identified proteins and PDAs were analyzed by InterPro, AnimalTFDB 4.0 and Cell-mPLOC 2.0 databases, respectively.

Results: A total of 8565 somatic proteins of adult T. canis were identified. Compared to male adult, 682 up-regulated PDAs and 844 down-regulated PDAs were identified in female adult with P-values < 0.05 and |log₂^FC| > 1, including 139 proteins exclusively expressed in female and 272 proteins exclusively expressed in male. The GO annotation analysis using all PDAs revealed that the main biological processes, cellular components and molecular functions corresponded to aminoglycan metabolic process, extracellular region and protein tyrosine phosphatase activity, respectively. The KEGG analysis using all PDAs showed that the pathways were mainly associated with adipocytokine signaling pathway, proximal tubule bicarbonate reclamation and PPAR signaling pathway.

Conclusions: This study reveals the differential protein expression between female and male adult T. canis, providing valuable resource for developing the novel intervention strategies against T. canis infection in humans and animals, especially from the perspective of sexual development and reproduction.

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使用 Orbitrap Astral 分析仪进行的蛋白质组学分析揭示了雌性和雄性成年犬毒蛾蛋白质表达的差异。

背景：犬弓形虫（Toxocara canis）是狗和其他犬科动物中最常见的蠕虫，也是具有重要社会经济意义的人畜共患寄生虫之一，尤其影响贫困社区的儿童和青少年群体。然而，有关雌性和雄性成年犬睾丸蠕虫蛋白质组的信息非常有限。为了填补这一知识空白，我们进行了一项全面的蛋白质组分析，以确定犬尾蝇蛆雌雄成虫之间丰度不同的蛋白质（PDAs）和性别特异性表达的蛋白质：方法：比较蛋白质组分析采用 Orbitrap 质谱（MS）和非对称无损跟踪（Astral）分析仪进行。采用 t 检验进行差异分析，并通过平行反应监测（PRM）对蛋白质进行验证。通过基因本体（Gene Ontology，GO）和京都基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）数据库预测了已鉴定的成年犬蛋白和 PDAs 的潜在生物学功能。通过InterPro、AnimalTFDB 4.0和Cell-mPLOC 2.0数据库分别分析了已鉴定蛋白质和PDA的结构域、转录因子和亚细胞定位：结果：共鉴定出8565个成年犬体细胞蛋白。与雄性成年犬相比，在雌性成年犬中发现了682个上调PDAs和844个下调PDAs，P值2FC|>1，其中139个蛋白质在雌性成年犬中独家表达，272个蛋白质在雄性成年犬中独家表达。利用所有 PDAs 进行的 GO 注释分析表明，主要的生物过程、细胞组分和分子功能分别与氨基聚糖代谢过程、细胞外区域和蛋白酪氨酸磷酸酶活性相对应。利用所有 PDA 进行的 KEGG 分析表明，这些通路主要与脂肪细胞因子信号通路、近端肾小管碳酸氢盐再生和 PPAR 信号通路相关：本研究揭示了雌性和雄性成年犬尾狮尾狒狒蛋白质表达的差异，尤其是从性发育和生殖的角度，为人类和动物开发新的犬尾狮尾狒狒感染干预策略提供了宝贵的资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infectious Diseases of Poverty INFECTIOUS DISEASES-

自引率

1.20%

发文量

368

期刊介绍： Infectious Diseases of Poverty is an open access, peer-reviewed journal that focuses on addressing essential public health questions related to infectious diseases of poverty. The journal covers a wide range of topics including the biology of pathogens and vectors, diagnosis and detection, treatment and case management, epidemiology and modeling, zoonotic hosts and animal reservoirs, control strategies and implementation, new technologies and application. It also considers the transdisciplinary or multisectoral effects on health systems, ecohealth, environmental management, and innovative technology. The journal aims to identify and assess research and information gaps that hinder progress towards new interventions for public health problems in the developing world. Additionally, it provides a platform for discussing these issues to advance research and evidence building for improved public health interventions in poor settings.