Genotypes of carboxypeptidase A1 and gamma-glutamyltransferase 1 may be useful tools for the diagnosis and the predictor of worrisome features of intraductal papillary mucinous neoplasm in Japan

IF 1.7 Q3 GASTROENTEROLOGY & HEPATOLOGY

JGH Open Pub Date : 2024-10-07 DOI:10.1002/jgh3.70031

Shuhei Agawa, Seiji Futagami, Ken Nakamura, Mayu Habiro, Rie Kawawa, Yuto Shinagawa, Rina Motomiya, Kumiko Kirita, Teppei Akimoto, Takeshi Onda, Tomohide Tanabe, Nobue Ueki, Kazufumi Honda, Kok-Ann Gwee, Katsuhiko Iwakiri

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引用次数: 0

Abstract

Background and Aim

This study aimed to clarify whether several single-nucleotide polymorphisms (SNPs)-related chronic pancreatitis such as carboxypeptidase A1 (CPA1), carboxypeptidase B1 (CPB1), Gamma-glutamyltransferase 1 (GGT1), G-protein-coupled receptor Class C Group 6 Member A (GPRC6A), and serine protease inhibitor, Kazal type 1 (SPINK-1) genotypes were associated with clinical characteristics of patients with intraductal papillary mucinous neoplasm (IPMN) and worrisome features of IPMN.

Methods

We enrolled 100 patients with IPMN and 116 patients as a control. Serum p-amylase, lipase, trypsin, phospholipase A2 (PLA2), and elastase-1 levels were measured. An Olympus EUS (GF-UCT 260) was used to perform endosonography in 100 patients with IPMN. Total EUS score was evaluated using endosonography. DNA was isolated from the duodenal tissue using a commercial system and polymerase chain reaction (PCR) was performed on 7500 Fast PCR System.

Results

There were no associations between glucose tolerances, lipid levels and genotypes of CPA1, GGT1, GPRC6A, and SPINK-1 in patients with IPMN. CPA1 genotype was significantly associated with the pathophysiology of IPMN. Then, GGT1 genotype was also significantly associated with EUS total score and the size of cyst more than 20 mm and more than 30 mm as one of worrisome features of IPMN.

Conclusion

Genotypes of carboxypeptidase A1 and gamma-glutamyltransferase 1 may be useful tools for the diagnosis and the predictor of worrisome features of IPMN.

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在日本，羧肽酶 A1 和γ-谷氨酰转移酶 1 的基因型可能是诊断和预测导管内乳头状黏液瘤令人担忧的特征的有用工具。

背景和目的：本研究旨在阐明几种单核苷酸多态性（SNPs）相关的慢性胰腺炎，如羧肽酶A1（CPA1），羧肽酶B1（CPB1），γ-谷氨酰转移酶1（GGT1）、GPRC6A）和丝氨酸蛋白酶抑制剂卡扎尔 1 型（SPINK-1）的基因型与导管内乳头状粘液瘤（IPMN）患者的临床特征和 IPMN 的令人担忧的特征相关。研究方法我们招募了 100 名 IPMN 患者和 116 名对照组患者。测量血清中 p-淀粉酶、脂肪酶、胰蛋白酶、磷脂酶 A2 (PLA2) 和弹性蛋白酶-1 的水平。使用奥林巴斯 EUS（GF-UCT 260）对 100 名 IPMN 患者进行内窥镜检查。通过内镜检查评估 EUS 总分。使用商用系统从十二指肠组织中分离出DNA，并在7500快速PCR系统上进行聚合酶链反应（PCR）：结果：IPMN 患者的葡萄糖耐量、血脂水平与 CPA1、GGT1、GPRC6A 和 SPINK-1 基因型之间没有关联。CPA1 基因型与 IPMN 的病理生理学显著相关。此外，GGT1 基因型与 EUS 总分以及 IPMN 的一个令人担忧的特征--囊肿大小超过 20 毫米和超过 30 毫米也有显著相关性：结论：羧肽酶 A1 和γ-谷氨酰转移酶 1 的基因型可能是诊断 IPMN 和预测 IPMN 危险特征的有用工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

JGH Open GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

3.40

自引率

0.00%

发文量

143

审稿时长

7 weeks