Blastocystis occurrence and subtype diversity in European wild boar (Sus scrofa) from the Iberian Peninsula.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Pamela C Köster, Ana M Figueiredo, Jenny G Maloney, Alejandro Dashti, Begoña Bailo, Rita T Torres, Carlos Fonseca, Atle Mysterud, Miguel Á Habela, Antonio Rivero-Juarez, Joaquín Vicente, Emmanuel Serrano, Maria C Arnal, Daniel Fernández de Luco, José A Armenteros, Ana Balseiro, Guillermo A Cardona, João Carvalho, Dário Hipólito, Joana Fernandes, Josman D Palmeira, Rafael Calero-Bernal, David González-Barrio, Monica Santin, David Carmena
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引用次数: 0

Abstract

The ongoing increase in wild boar populations across Europe has fostered human-wildlife conflicts, including the transmission of emerging pathogens with zoonotic importance. Blastocystis is a ubiquitous, faecal-oral transmitted protist that can cause gastrointestinal illnesses and is observed in humans and animals worldwide. The role of wildlife in the epidemiology of Blastocystis is insufficiently understood. Thus, we investigated the occurrence and subtype diversity of Blastocystis in free-ranging wild boars from the Iberian Peninsula using conventional PCR and next-generation amplicon sequencing of a fragment of the ssu RNA gene. A total of 459 wild boar faecal samples were collected across Spain (n = 360) and Portugal (n = 99) between 2014 and 2021. Blastocystis was present in 15.3% (70/459; 95% CI 12.1-18.9) of the wild boars analysed, and its occurrence was significantly higher in Portugal (34.3%, 34/99; 95% CI 25.1-44.6) than in Spain (10.0%, 36/360; 95% CI 7.1-13.6). Seven Blastocystis subtypes (ST5, ST10b, ST13-ST15, ST24b, and ST43) were detected among the surveyed wild boar populations, with greater variability detected in Portuguese samples. ST5 was identified in all the Blastocystis-positive animals, whereas 14.3% of them harboured ST mixed colonisations. Our results demonstrate that Blastocystis ST5 is particularly adapted to infect wild boars. The additional identification of zoonotic STs reinforces the role of wild boars as spreaders of zoonotic infections with public health significance.

伊比利亚半岛欧洲野猪(Sus scrofa)中的布氏囊虫发生率和亚型多样性。
欧洲各地野猪数量的持续增长引发了人类与野生动物之间的冲突,包括具有人畜共患病重要性的新病原体的传播。Blastocystis 是一种无处不在、经粪口传播的原生动物,可引起胃肠道疾病,在世界各地的人类和动物中都可见到。人们对野生动物在布氏囊虫流行病学中的作用了解不足。因此,我们采用传统的 PCR 技术和 ssu RNA 基因片段的下一代扩增片段测序技术,调查了伊比利亚半岛散养野猪中布氏囊尾蚴的发生率和亚型多样性。2014 年至 2021 年期间,在西班牙(n = 360)和葡萄牙(n = 99)共收集了 459 份野猪粪便样本。在所分析的野猪中,15.3%(70/459;95% CI 12.1-18.9)的野猪体内存在囊尾蚴,葡萄牙的发生率(34.3%,34/99;95% CI 25.1-44.6)明显高于西班牙(10.0%,36/360;95% CI 7.1-13.6)。在所调查的野猪种群中检测到了七种囊泡亚型(ST5、ST10b、ST13-ST15、ST24b 和 ST43),在葡萄牙样本中检测到的变异性更大。在所有囊泡菌阳性动物中都发现了 ST5 型,而其中 14.3% 的动物带有 ST 混合型菌落。我们的研究结果表明,ST5 型布氏杆菌特别适合感染野猪。人畜共患病 ST 的额外鉴定加强了野猪作为具有公共卫生意义的人畜共患病传播者的作用。
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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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