Impacts of dietary supplementation of chitosan nanoparticles on growth, carcass traits nutrient digestibility, blood biochemistry, intestinal microbial load, and meat quality of broilers.

Abstract

This study explores the impact of chitosan nanoparticles (CNP) on the performance, nutrient digestibility, blood biochemical, immunity, microbial load, carcass traits, and meat attributes of broilers. A total of 200 7-d-old Cobb chicks were distributed to 4 groups, each replicated 5 times, with 10 birds in each replicate. The experimental diets were as follows: First group was fed a basal diet only (control); 2nd, 3rd, and 4th groups received a basal diet supplemented with 0.2, 0.3, and 0.4 g CNP/kg of feed, respectively. Results showed that the body weight (BW) and body weight gain significantly improved (P < 0.05) in the birds belonging to the 0.4 CNP group compared to the other groups. The best feed efficiency (feed conversion ratio [FCR]) was found in the group supplemented with a 0.4-g CNP/kg diet. The digestibility coefficients for dry matter and crude protein were significantly higher, and ether extract was significantly lower in the 0.4 g CNP/kg group than in other groups (P < 0.05). Broiler birds of the 0.4 CNP group had significantly (P < 0.05) reduced serum cholesterol, AST, and ALT levels. The humoral immunity (increased serum IgG and IgM levels) tended to improve in birds fed 0.3 and 0.4 g CNP/kg of feed. Compared to the control, total bacterial load and coliform count decreased significantly (P < 0.05) by supplementing 0.4 g CNP in the diet. The dressing weight, breast weight, and abdominal fat % were altered in birds receiving dietary 0.4 g CNP/kg. The treatment with CNP at 0.4 g/kg feed enhanced the broiler meat quality by increasing the values for water holding capacity, ABTS [2, 2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)], DPPH (2,2-diphenyl-1-picrylhydrazyl) while reducing the thiobarbituric acid reactive substances (TBARS) value. Based on the results above, it could be concluded that CNP supplementation at 0.4 g/kg is recommended as a beneficial feed additive for broiler chickens.