Antibacterial performance of nanosecond laser irradiated zirconium-based bulk metallic glass.

IF 1.7 4区 医学 Q3 ENGINEERING, BIOMEDICAL
Cezhi Du, Chengyong Wang, Jianbo Sui, Lijuan Zheng
{"title":"Antibacterial performance of nanosecond laser irradiated zirconium-based bulk metallic glass.","authors":"Cezhi Du, Chengyong Wang, Jianbo Sui, Lijuan Zheng","doi":"10.1177/09544119241285659","DOIUrl":null,"url":null,"abstract":"<p><p>Bulk metallic glasses (BMGs) have garnered significant attention in recent decades due to the outstanding physical, chemical, and biomedical characteristics. The biomedical application of metallic glass also received extensive attention. This report investigates the interplay among antibacterial performance, crystallization and processing parameters of Zr-based bulk metallic glass (Zr-BMG) following nanosecond laser irradiation. We examined surface morphology, crystallization behavior, surface quality, binding energy, and ion release properties post-laser irradiation. Additionally, we evaluated the generation of reactive oxygen species upon immersion of Zr-BMG in phosphate-buffered saline using the 2',7'-dichlorofluorescin diacetate method. Staphylococcus aureus was chosen to assess Zr-BMG's antibacterial performance, while mouse osteoblasts were utilized to investigate in vitro cytotoxicity. Our findings revealed that at laser energy intensities below 0.08 J/mm<sup>2</sup>, the amorphous structure of Zr-BMG remained intact after irradiation. Moreover, laser irradiation significantly enhanced the antibacterial performance of Zr-BMG. The release rate of ion, concentration of reactive oxygen species, and antibacterial properties exhibited direct proportionality to laser energy intensity. However, surfaces exhibiting high antibacterial efficacy also displayed elevated cytotoxicity. The surface irradiated with a 7 μJ ablation pulse and 200 mm/s irradiation speed demonstrated a superior balance between antibacterial and cytotoxic properties while maintaining an amorphous state. We hope this research can provide theoretical reference and data support for the application of metallic glass in biomedical application.</p>","PeriodicalId":20666,"journal":{"name":"Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/09544119241285659","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/8 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Bulk metallic glasses (BMGs) have garnered significant attention in recent decades due to the outstanding physical, chemical, and biomedical characteristics. The biomedical application of metallic glass also received extensive attention. This report investigates the interplay among antibacterial performance, crystallization and processing parameters of Zr-based bulk metallic glass (Zr-BMG) following nanosecond laser irradiation. We examined surface morphology, crystallization behavior, surface quality, binding energy, and ion release properties post-laser irradiation. Additionally, we evaluated the generation of reactive oxygen species upon immersion of Zr-BMG in phosphate-buffered saline using the 2',7'-dichlorofluorescin diacetate method. Staphylococcus aureus was chosen to assess Zr-BMG's antibacterial performance, while mouse osteoblasts were utilized to investigate in vitro cytotoxicity. Our findings revealed that at laser energy intensities below 0.08 J/mm2, the amorphous structure of Zr-BMG remained intact after irradiation. Moreover, laser irradiation significantly enhanced the antibacterial performance of Zr-BMG. The release rate of ion, concentration of reactive oxygen species, and antibacterial properties exhibited direct proportionality to laser energy intensity. However, surfaces exhibiting high antibacterial efficacy also displayed elevated cytotoxicity. The surface irradiated with a 7 μJ ablation pulse and 200 mm/s irradiation speed demonstrated a superior balance between antibacterial and cytotoxic properties while maintaining an amorphous state. We hope this research can provide theoretical reference and data support for the application of metallic glass in biomedical application.

纳秒激光辐照锆基块状金属玻璃的抗菌性能。
近几十年来,块状金属玻璃(BMGs)因其出色的物理、化学和生物医学特性而备受关注。金属玻璃的生物医学应用也受到广泛关注。本报告研究了纳秒激光辐照后锆基块状金属玻璃(Zr-BMG)的抗菌性能、结晶和加工参数之间的相互作用。我们考察了激光辐照后的表面形态、结晶行为、表面质量、结合能和离子释放特性。此外,我们还使用 2',7'-二氯荧光素二乙酸酯法评估了 Zr-BMG 浸入磷酸盐缓冲盐水后活性氧的生成情况。我们选择金黄色葡萄球菌来评估 Zr-BMG 的抗菌性能,并利用小鼠成骨细胞来研究体外细胞毒性。我们的研究结果表明,当激光能量强度低于 0.08 J/mm2 时,Zr-BMG 的无定形结构在辐照后保持不变。此外,激光辐照大大提高了 Zr-BMG 的抗菌性能。离子释放率、活性氧浓度和抗菌性能与激光能量强度成正比。然而,具有高抗菌功效的表面同时也显示出较高的细胞毒性。以 7 μJ 的烧蚀脉冲和 200 mm/s 的辐照速度辐照的表面在保持无定形状态的同时,在抗菌性和细胞毒性之间实现了极佳的平衡。我们希望这项研究能为金属玻璃在生物医学领域的应用提供理论参考和数据支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
3.60
自引率
5.60%
发文量
122
审稿时长
6 months
期刊介绍: The Journal of Engineering in Medicine is an interdisciplinary journal encompassing all aspects of engineering in medicine. The Journal is a vital tool for maintaining an understanding of the newest techniques and research in medical engineering.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信