Impact of estradiol in inducing endometrial cancer using RL95-2

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2024-10-03 DOI:10.1016/j.prp.2024.155640

Anuja Pant, Kareena Moar, Pawan Kumar Maurya

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Abstract

Background

Endometrial cancer is the most common gynecological malignancy that originates from the inner lining of the uterus and predominantly affects postmenopausal women. Prolonged exposure to estrogen, family history of endometrial cancer, obesity, and hormonal imbalance are some of the risk factors associated with endometrial cancer. In our study, we investigated the effect of estradiol, a potent form of estrogen at various concentrations on endometrial cell line RL95–2.

Methods

Endometrial cell RL95–2 were cultured in DMEM medium with optimal conditions required to maintain the cells. MTT assay and colony formation assay were further performed after treating the cells with different concentrations of estradiol (1, 10, and 100 nM) and TAM (100 nM). Moreover, the effect of genes regulated by estradiol was also examined using microarray and validated using real-time polymerase chain reaction (qRT-PCR).

Results

Time-dependent MTT assay shows a significant change in the ability of the cells to survive relative to concentrations. Colony formation was found to be directly proportional to the concentration of the estradiol (p < 0.05). Among genes, MMP14 (p = 0.03), SPARCL1 (p = 0.005), and CLU (p = 0.06) showed a significant up-regulation in their expression after estradiol treatment while NRN1 (p < 0.001) showed significant downregulation in expression pattern compared to control. However, the TAM treatment was found to be significantly effective after 72 h (p < 0.001) compared to control and 100 nM E2 (p = 0.0206).

Conclusion

Our study suggests that estradiol significantly contributes to regulating the viability, colony formation, and expression of genes associated with endometrial cancer.

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雌二醇对使用 RL95-2 诱导子宫内膜癌的影响

背景：子宫内膜癌是最常见的妇科恶性肿瘤，起源于子宫内膜，主要影响绝经后妇女。长期接触雌激素、子宫内膜癌家族史、肥胖和内分泌失调是与子宫内膜癌相关的一些风险因素。在我们的研究中，我们调查了不同浓度的雌二醇（一种强效雌激素）对子宫内膜细胞株 RL95-2 的影响：方法：将子宫内膜细胞 RL95-2 培养在 DMEM 培养基中，以维持细胞所需的最佳条件进行培养。用不同浓度的雌二醇（1、10 和 100 nM）和 TAM（100 nM）处理细胞后，进一步进行 MTT 检测和集落形成检测。此外，还使用芯片检测了雌二醇调控基因的影响，并使用实时聚合酶链反应（qRT-PCR）进行了验证：结果：时间依赖性 MTT 检测显示，相对于浓度，细胞的存活能力发生了显著变化。发现菌落的形成与雌二醇的浓度成正比（p < 0.05）。在基因中，MMP14（p = 0.03）、SPARCL1（p = 0.005）和 CLU（p = 0.06）的表达在雌二醇处理后显著上调，而 NRN1（p < 0.001）的表达模式与对照组相比显著下调。然而，与对照组和 100 nM E2（p = 0.0206）相比，72 h 后发现 TAM 处理明显有效（p < 0.001）：我们的研究表明，雌二醇对子宫内膜癌相关基因的活力、集落形成和表达有明显的调节作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.