SENP7 inhibits glioblastoma metastasis and invasion by dissociating SUMO2/3 binding to specific target proteins.

IF 1.7 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Open Medicine Pub Date : 2024-10-07 eCollection Date: 2024-01-01 DOI:10.1515/med-2024-1052
Jixing Zhang, Hongshan Zheng, Peng Liang
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引用次数: 0

Abstract

Background: The poor surgical efficacy and recurrence of glioblastoma (GBM) are due to its lack of visible infiltrative features. Our bioinformatics study suggests that low expression of small ubiquitin-like modifier (SUMO)-specific protease 7 (SENP7) indicates poor prognosis in GBM.

Objectives: This study investigated the effect of SENP7 expression on the invasion, migration, and proliferation of GBM cells and aims to identify the SUMO target proteins affected by SENP7.

Methods: SENP7 expression was analyzed in eight GBM tumor samples and four GBM cell lines, comparing them to normal brain tissue. The effect of SENP7 overexpression on GBM LN229 cell migration, invasion, and proliferation was examined through in vitro assays. Furthermore, four SUMO target proteins involved in tumor invasion and proliferation (CDK6, matrix metalloproteinase-9 [MMP9], AKT, and HIF-1α) were studied to explore SENP7's molecular mechanism.

Results: SENP7 expression was significantly lower in GBM tumors compared to normal tissue. SENP7 overexpression in LN229 cells inhibited migration and invasion without affecting proliferation. Overexpression reduced the levels of MMP9, AKT, and HIF-1α, but not CDK6. Immunohistochemical analysis showed decreased MMP9 and CD31 levels, suggesting reduced tumor invasion and angiogenesis. However, SENP7 overexpression did not affect tumor growth in vivo.

Conclusions: SENP7 inhibits GBM invasion by dissociating proteins associated with tumor invasion from SUMO2/3, providing a potential target for future GBM therapies.

SENP7 通过分离 SUMO2/3 与特定靶蛋白的结合,抑制胶质母细胞瘤的转移和侵袭。
背景:胶质母细胞瘤(GBM)的手术疗效差、复发率高,这是因为它缺乏明显的浸润特征。我们的生物信息学研究表明,小泛素样修饰物(SUMO)特异性蛋白酶7(SENP7)的低表达预示着GBM的不良预后:本研究调查了SENP7表达对GBM细胞侵袭、迁移和增殖的影响,并旨在确定受SENP7影响的SUMO靶蛋白:方法:分析了 8 个 GBM 肿瘤样本和 4 个 GBM 细胞系中 SENP7 的表达情况,并将它们与正常脑组织进行了比较。通过体外实验检测了 SENP7 过表达对 GBM LN229 细胞迁移、侵袭和增殖的影响。此外,还研究了参与肿瘤侵袭和增殖的四个 SUMO 靶蛋白(CDK6、基质金属蛋白酶-9 [MMP9]、AKT 和 HIF-1α),以探索 SENP7 的分子机制:结果:SENP7在GBM肿瘤中的表达明显低于正常组织。SENP7在LN229细胞中的过表达可抑制迁移和侵袭,但不影响增殖。过表达会降低 MMP9、AKT 和 HIF-1α 的水平,但不会降低 CDK6 的水平。免疫组化分析显示,MMP9 和 CD31 水平降低,表明肿瘤侵袭和血管生成减少。然而,SENP7的过表达并不影响肿瘤在体内的生长:结论:SENP7 通过使与肿瘤侵袭相关的蛋白与 SUMO2/3 分离来抑制 GBM 的侵袭,为未来的 GBM 治疗提供了一个潜在的靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Open Medicine
Open Medicine Medicine-General Medicine
CiteScore
3.00
自引率
0.00%
发文量
153
审稿时长
20 weeks
期刊介绍: Open Medicine is an open access journal that provides users with free, instant, and continued access to all content worldwide. The primary goal of the journal has always been a focus on maintaining the high quality of its published content. Its mission is to facilitate the exchange of ideas between medical science researchers from different countries. Papers connected to all fields of medicine and public health are welcomed. Open Medicine accepts submissions of research articles, reviews, case reports, letters to editor and book reviews.
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