Mapping voltage activity in a live mouse brain in 3D using confocal light field microscopy

IF 36.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2024-10-08 DOI:10.1038/s41592-024-02459-4

引用次数: 0

Abstract

Voltage imaging, a promising technique for directly recording neuronal activity, faces barriers to broad application due to current limitations in compatible imaging modalities. Our team introduces an advanced confocal light field microscopy method enabling high-throughput, rapid and low-noise 3D voltage imaging in awake mice.

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利用共聚焦光场显微镜绘制活体小鼠大脑的三维电压活动图。

电压成像是一种很有前途的直接记录神经元活动的技术，但由于目前兼容成像模式的限制，该技术的广泛应用面临障碍。我们的团队引入了一种先进的共聚焦光场显微镜方法，可在清醒小鼠体内实现高通量、快速和低噪声的三维电压成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.