Evaluation of Microbial Phospholipase and Lipase Activity Through the Chromatographic Analysis of Crude, Degummed, and Transesterified Soybean Oil for Biodiesel Production

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Ahlem Eddehech, Alessia Tropea, Francesca Rigano, Danilo Donnarumma, Emna Ben Abdallah, Francesco Cacciola, Luigi Mondello, Zied Zarai
{"title":"Evaluation of Microbial Phospholipase and Lipase Activity Through the Chromatographic Analysis of Crude, Degummed, and Transesterified Soybean Oil for Biodiesel Production","authors":"Ahlem Eddehech,&nbsp;Alessia Tropea,&nbsp;Francesca Rigano,&nbsp;Danilo Donnarumma,&nbsp;Emna Ben Abdallah,&nbsp;Francesco Cacciola,&nbsp;Luigi Mondello,&nbsp;Zied Zarai","doi":"10.1002/jssc.202400325","DOIUrl":null,"url":null,"abstract":"<p>The present study aimed at synthesizing fatty acid methyl esters in a combined enzymatic method by applying degumming and transesterification of soybean oil. A soluble lipase from <i>Serratia</i> sp. W3 and a recombinant phosphatidylcholine-preferring phospholipase C (PC-PLC) from <i>Bacillus thuringiensis</i> were used in a consecutive manner for phosphorus removal and conversion into methyl esters. By applying 1% of recombinant PC-PLC almost 83% of phosphorus was removed (final content of 21.01 mg/kg). Moreover, a sensitive and selective high-performance liquid chromatography method coupled to tandem mass spectrometry was applied to obtain a comprehensive lipid profile for the simultaneous evaluation of phospholipids removal and diacylglycerol (DAG) increase. A significant increase for all the monitored DAG species, up to 138.42%, was observed by using the enzymatic degumming, in comparison to the crude sample, resulting in an increased oil yield. <i>Serratia</i> sp. W3 lipase was identified as a suitable biocatalyst for biodiesel production, converting efficiently the acylglycerols. The results regarding the physical-chemical characteristics show that the cetane level, density and pour point of the obtained biodiesel are close to current regulation requirements. These findings highlight the potential of a two-step process implementation, based on the combination of lipase and phospholipase, as a suitable alternative for biodiesel production.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.202400325","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of separation science","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jssc.202400325","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

The present study aimed at synthesizing fatty acid methyl esters in a combined enzymatic method by applying degumming and transesterification of soybean oil. A soluble lipase from Serratia sp. W3 and a recombinant phosphatidylcholine-preferring phospholipase C (PC-PLC) from Bacillus thuringiensis were used in a consecutive manner for phosphorus removal and conversion into methyl esters. By applying 1% of recombinant PC-PLC almost 83% of phosphorus was removed (final content of 21.01 mg/kg). Moreover, a sensitive and selective high-performance liquid chromatography method coupled to tandem mass spectrometry was applied to obtain a comprehensive lipid profile for the simultaneous evaluation of phospholipids removal and diacylglycerol (DAG) increase. A significant increase for all the monitored DAG species, up to 138.42%, was observed by using the enzymatic degumming, in comparison to the crude sample, resulting in an increased oil yield. Serratia sp. W3 lipase was identified as a suitable biocatalyst for biodiesel production, converting efficiently the acylglycerols. The results regarding the physical-chemical characteristics show that the cetane level, density and pour point of the obtained biodiesel are close to current regulation requirements. These findings highlight the potential of a two-step process implementation, based on the combination of lipase and phospholipase, as a suitable alternative for biodiesel production.

通过对用于生物柴油生产的粗豆油、脱胶豆油和酯交换豆油进行色谱分析,评估微生物磷脂酶和脂肪酶的活性。
本研究旨在通过对大豆油进行脱胶和酯交换反应,采用联合酶法合成脂肪酸甲酯。连续使用来自 Serratia sp. W3 的可溶性脂肪酶和来自苏云金芽孢杆菌的重组磷脂酰胆碱偏爱磷脂酶 C (PC-PLC)进行除磷和转化为甲酯。使用 1%的重组 PC-PLC 可以去除近 83% 的磷(最终含量为 21.01 mg/kg)。此外,为了同时评估磷脂的去除和二酰甘油(DAG)的增加情况,还采用了一种灵敏且选择性强的高效液相色谱法和串联质谱法,以获得全面的脂质概况。与粗样品相比,使用酶法脱胶后,所有监测到的 DAG 种类都明显增加,最高达 138.42%,从而提高了产油量。经鉴定,沙雷氏菌 W3 脂肪酶是一种适用于生物柴油生产的生物催化剂,可有效转化酰基甘油。有关物理化学特性的研究结果表明,所获得生物柴油的十六烷值、密度和倾点均接近现行法规的要求。这些结果凸显了基于脂肪酶和磷脂酶组合的两步法作为生物柴油生产的合适替代方法的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信