TGF-β2, EGF and FGF21 influence the suckling rat intestinal maturation

IF 4.8 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Nutritional Biochemistry Pub Date : 2024-10-05 DOI:10.1016/j.jnutbio.2024.109778

Blanca Grases-Pintó , Paulina Torres-Castro , Mar Abril-Gil , Margarida Castell , María J. Rodríguez-Lagunas , Francisco J. Pérez-Cano , Àngels Franch

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Abstract

Some of the growth factors present in breast milk, such as transforming growth factor-β (TGF-β), epidermal growth factor (EGF) and fibroblast growth factor 21 (FGF21), play important roles in the development of the intestinal tract. The aim of this study was to determine the effect of a supplementation with TGF-β2, EGF and FGF21 on suckling rats intestinal maturation. For this purpose, Wistar rats were supplemented daily with TGF-β2, EGF or FGF21 throughout the suckling period. We evaluated the functionality of the intestinal epithelial barrier through an in vivo dextran permeability assay, and by a histomorphometric and immunohistochemical study. In addition, the intestinal gene expression of tight junction-associated proteins, mucins, toll-like receptors, and maturation markers was analyzed. Moreover, the intraepithelial lymphocyte (IEL) phenotypical composition was established. During the suckling period, the supplementation with TGF-β2, EGF and FGF21 showed important signs of intestinal maturation. These results suggest that these molecules, present in breast milk, play a modulatory role in the maturation of the intestinal barrier function and the IEL composition during the suckling period.

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TGF-β2、EGF 和 FGF21 对乳鼠肠道成熟的影响

母乳中的一些生长因子，如转化生长因子-β（TGF-β）、表皮生长因子（EGF）和成纤维细胞生长因子 21（FGF21），在肠道发育中发挥着重要作用。本研究旨在确定补充 TGF-β2、EGF 和 FGF21 对乳鼠肠道成熟的影响。为此，我们在 Wistar 大鼠的整个哺乳期每天为其补充 TGF-β2、EGF 或 FGF21。我们通过体内葡聚糖渗透性试验以及组织形态学和免疫组化研究评估了肠上皮屏障的功能。此外，还分析了肠道紧密连接相关蛋白、粘蛋白、收费样受体和成熟标志物的基因表达。此外，还确定了上皮内淋巴细胞（IEL）的表型组成。在哺乳期，补充 TGF-β2、EGF 和 FGF21 可显示肠道成熟的重要迹象。这些结果表明，母乳中的这些分子对哺乳期肠道屏障功能和IEL组成的成熟起着调节作用。

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来源期刊

Journal of Nutritional Biochemistry 医学-生化与分子生物学

CiteScore

9.50

自引率

3.60%

发文量

237

审稿时长

68 days

期刊介绍： Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.