Histopathological and biochemical evaluation of the protective efficacy of Prunus spinosa L. extract in a rat model of indomethacin-induced gastric ulcer.

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Nihal Cetin, Esma Menevse, Cengizhan Ceylan, Zeliha Esin Celik, Neriman Akdam, Seyma Tetik Rama, Tugsen Buyukyildirim, Leyla Pasayeva, Osman Tugay, Meltem Gumus
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引用次数: 0

Abstract

Objectives: Some species of Prunus L. are popularly used to treat gastric ulcers. However, the possible healing mechanisms of the anti-ulcer activity of P. spinosa, which has proven antioxidant, anti-inflammatory, and wound-healing properties, are unclear.

Materials and methods: Ethanol extracts of P. spinosa fruits were administered orally at 100 mg/kg and 200 mg/kg to Wistar albino rats, with an indomethacin-induced gastric ulcer model. The ulcerous areas on the stomach surface were examined macroscopically. Tissues were examined histopathologically and biochemically. LC-HRMS revealed the phytochemical content.

Results: TNF-α, IL-6, IL-1β, IL-8, and NF-kB levels were higher in the gastric ulcer group than in the extract groups. The VEGF values did not differ in each group. A significant difference was found between the lansoprazole group and the high-dose P. spinosa group regarding PGE2 levels. A histopathologically significant difference was observed between the healthy group and the indomethacin-applied groups in terms of neutrophilic infiltration of the gastric mucosa. Ascorbic acid (1547.521 µg/g), homoprotocatechuic acid (1268.217 µg/g), and genistein (1014.462 µg/g) were found as the main compounds in the P. spinosa extract by LC-HRMS.

Conclusion: Our results demonstrated that P. spinosa protected the gastric mucosa from inflammation and also modulated the PGE2 pathway. When considered in terms of TNF-α, IL-1β, IL-8, IL-6, PGE2, and NF-kB values, it can be concluded that it has a similar or even more positive effect than the reference substance. P. spinosa showed its effects in a dose-dependent manner.

对刺李提取物在吲哚美辛诱导的胃溃疡大鼠模型中的保护功效进行组织病理学和生物化学评估。
目的:刺五加的一些品种被广泛用于治疗胃溃疡。然而,被证明具有抗氧化、抗炎和伤口愈合特性的刺李的抗溃疡活性的可能愈合机制尚不清楚:以吲哚美辛诱导的胃溃疡模型为研究对象,分别给 Wistar 白化大鼠口服 100 毫克/千克和 200 毫克/千克的刺五加果实乙醇提取物。对胃表面的溃疡区进行宏观检查。组织病理学和生物化学检查。LC-HRMS显示了植物化学成分的含量:结果:胃溃疡组的 TNF-α、IL-6、IL-1β、IL-8 和 NF-kB 水平高于提取物组。各组的 VEGF 值没有差异。在 PGE2 水平方面,兰索拉唑组和高剂量刺五加组之间存在明显差异。在胃黏膜的中性粒细胞浸润方面,健康组和吲哚美辛应用组之间存在组织病理学上的显著差异。通过 LC-HRMS 分析发现,抗坏血酸(1547.521 µg/g)、高儿茶酸(1268.217 µg/g)和染料木素(1014.462 µg/g)是刺五加提取物中的主要化合物:我们的研究结果表明,刺五加能保护胃黏膜免受炎症侵袭,还能调节 PGE2 通路。从 TNF-α、IL-1β、IL-8、IL-6、PGE2 和 NF-kB 值的角度考虑,可以得出结论:刺五加具有与参考物质相似甚至更积极的作用。刺五加的作用具有剂量依赖性。
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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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