The Role of Bovine Amniotic Membrane and Hydroxyapatite for the Ridge Preservation.

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
International Journal of Biomaterials Pub Date : 2024-09-14 eCollection Date: 2024-01-01 DOI:10.1155/2024/4053527
Octarina Octarina, Elly Munadziroh, Fathilah Abdul Razak, Ekowati Handharyani, Meircurius Dwi Condro Surboyo
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引用次数: 0

Abstract

Ridge preservation is an important technique for maintaining the dimensions of the alveolar bone following tooth extraction, which is crucial for successful tooth rehabilitation. The combination of bovine amniotic membrane and hydroxyapatite has shown promise as a scaffold material containing growth factors that can stimulate osteogenic-related factors such as bone morphogenetic protein 2 (BMP2), Runt-related transcription factor 2 (RUNX2), and osteocalcin. This stimulation leads to collagen production and osteoblast proliferation, resulting in new bone formation. In this study, bovine amniotic membrane-hydroxyapatite (BAM-HA) composites were prepared using three different ratios of bovine amniotic membrane and hydroxyapatite (2 : 3, 3 : 7, 7 : 13). Thirty Sprague-Dawley rats had their first incisors extracted, and different types of BAM-HA were applied for ridge preservation. The control group received no treatment, while the positive control group was given xenograft. After 14 and 28 days, the animals were sacrificed, and immunohistochemical analysis was performed to evaluate the expression of BMP2, RUNX2, and osteocalcin. Additionally, a histological examination was conducted to analyse collagen thickness and osteoblast cell proliferation. The results demonstrated that the application of BAM-HA significantly increased collagen density, osteoblast cell proliferation, and the expression of BMP2, RUNX2, and osteoclacin compared to the control group (p < 0.05) on both days 14 and 28. Furthermore, increasing the hydroxyapatite content in the composite was found to enhance collagen thickness, osteoblast cell proliferation, and the expression of osteogenic-related factors. These preliminary findings suggest that the combination of BAM-HA can be used for ridge preservation to prevent further bone resorption following tooth extraction.

牛羊膜和羟基磷灰石在牙脊保存中的作用
牙槽骨嵴保留是拔牙后保持牙槽骨尺寸的一项重要技术,这对于成功的牙齿修复至关重要。牛羊膜和羟基磷灰石的结合已显示出作为一种支架材料的前景,这种材料含有生长因子,能刺激骨形态发生蛋白 2(BMP2)、Runt 相关转录因子 2(RUNX2)和骨钙素等成骨相关因子。这种刺激会导致胶原蛋白生成和成骨细胞增殖,从而形成新骨。本研究使用三种不同比例的牛羊膜和羟基磷灰石(2 : 3、3 : 7、7 : 13)制备了牛羊膜-羟基磷灰石(BAM-HA)复合材料。30 只 Sprague-Dawley 大鼠拔出了第一颗门牙,并使用不同类型的 BAM-HA 进行牙脊保存。对照组不接受任何治疗,而阳性对照组则接受异种移植。14 天和 28 天后,动物被处死,并进行免疫组化分析,以评估 BMP2、RUNX2 和骨钙素的表达。此外,还进行了组织学检查,以分析胶原蛋白厚度和成骨细胞增殖情况。结果表明,与对照组相比,BAM-HA 的应用在第 14 天和第 28 天明显增加了胶原蛋白密度、成骨细胞增殖以及 BMP2、RUNX2 和骨钙素的表达(p < 0.05)。此外,研究还发现,增加复合材料中羟基磷灰石的含量可增强胶原蛋白厚度、成骨细胞增殖和成骨相关因子的表达。这些初步研究结果表明,BAM-HA 的组合可用于牙脊保存,防止拔牙后骨的进一步吸收。
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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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