The Immune Regulatory Functions in B Cells Are Restored by CpG to Reduce Experimental Food Allergy.

IF 4.9 3区 医学 Q2 IMMUNOLOGY
Immunology Pub Date : 2025-01-01 Epub Date: 2024-10-09 DOI:10.1111/imm.13868
Qiao Liu, Dong-Hua Bin, Zhuo-Ya Wang, Ke-Ping Peng, Wang Tang, Jing-Weng Huang, Ling-Zhi Xu, Xiang-Yu Wang, Ping-Chang Yang, Gui-Xiang Tian
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引用次数: 0

Abstract

Dysfunctional immune regulation contributes to the pathogenesis of food allergy (FA). The mechanism behind regulatory B-cell dysfunction is unclear. CpG has immune regulatory functions. The purpose of this study is to use CpG to recover the immune suppressive functions of B cells in mice with FA. An FA mouse model was created using ovalbumin as the specific antigen. Flow cytometry was used to isolate B cells from the intestinal tissues. The immune regulatory functions of B cells were assessed using immunological approaches. The results showed that the FA response was linked to low IL-10 levels in gut lavage fluids of FA mice. FA mouse intestinal B cells produced lower amounts of IL-10 as compared with B cells isolated from naïve control mice. Impaired immune suppressive functions were observed in B cells isolated from the FA mouse intestine. The inducibility of the Il10 expression in naïve B cells of the intestine of FA mice was defective. The induction of Il10 expression in FA B cells could be restored by CpG through regulating the methylation status of the Cmip promoter. CpG promoted the therapeutic efficacy of allergen specific immunotherapy by restoring the induction of IL-10+ B cells in the intestine. The expression of Il10 in B cells of the FA mouse intestine was impaired. Administration of CpG could restore the expression of Il10 in B cells in the intestine and promote immunotherapy for FA.

CpG可恢复B细胞的免疫调节功能,减轻实验性食物过敏。
免疫调节功能失调是食物过敏(FA)的发病机制之一。调节性 B 细胞功能失调的机制尚不清楚。CpG具有免疫调节功能。本研究的目的是利用 CpG 恢复 FA 小鼠 B 细胞的免疫抑制功能。以卵清蛋白为特异性抗原建立了一个 FA 小鼠模型。使用流式细胞术从肠道组织中分离出 B 细胞。使用免疫学方法评估了 B 细胞的免疫调节功能。结果显示,FA反应与FA小鼠肠道灌洗液中低IL-10水平有关。与分离自天真对照组小鼠的 B 细胞相比,FA 小鼠肠道 B 细胞产生的 IL-10 含量较低。从FA小鼠肠道分离的B细胞的免疫抑制功能受损。在FA小鼠肠道的幼稚B细胞中,Il10表达的诱导性存在缺陷。CpG可以通过调节Cmip启动子的甲基化状态来恢复FA B细胞中Il10的诱导表达。CpG通过恢复肠道中IL-10+ B细胞的诱导,促进了过敏原特异性免疫疗法的疗效。FA小鼠肠道B细胞中Il10的表达受损。服用CpG可恢复肠道B细胞中Il10的表达,促进FA的免疫治疗。
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来源期刊
Immunology
Immunology 医学-免疫学
CiteScore
11.90
自引率
1.60%
发文量
175
审稿时长
4-8 weeks
期刊介绍: Immunology is one of the longest-established immunology journals and is recognised as one of the leading journals in its field. We have global representation in authors, editors and reviewers. Immunology publishes papers describing original findings in all areas of cellular and molecular immunology. High-quality original articles describing mechanistic insights into fundamental aspects of the immune system are welcome. Topics of interest to the journal include: immune cell development, cancer immunology, systems immunology/omics and informatics, inflammation, immunometabolism, immunology of infection, microbiota and immunity, mucosal immunology, and neuroimmunology. The journal also publishes commissioned review articles on subjects of topical interest to immunologists, and commissions in-depth review series: themed sets of review articles which take a 360° view of select topics at the heart of immunological research.
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