The Antibacterial Efficacy and Mechanism of Tea Polyphenol Against Drug-Resistant Aeromonas veronii TH0426 In Vitro.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Liying Peng, Zongtao Chen, Yanting Hei, Wenyan Wei, Defang Chen
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Abstract

The emergence of Motile Aeromonas Septicemia (MAS) caused by Aeromonas veronii in sturgeon farming has become a significant concern due to its high mortality impact on the aquaculture industry. The threat posed by MAS highlights the urgent need for effective control measures to combat bacterial infections in sturgeon populations. Tea polyphenol (TP) has demonstrated promising antibacterial properties against livestock and poultry bacterial infections. However, its antibacterial efficacy and mechanism in bacterial diseases of aquatic animals remain largely unexplored. This study aimed to investigate the in vitro antibacterial effect and mechanism of TP on fish-borne drug-resistant A. veronii TH0426 by assessing the impact of TP on TH0426 cell growth, antibiofilm activity, morphology, as well as measuring electrical conductivity, DNA extravasation, lactate dehydrogenase (LDH) activity, protein, and DNA contents. Results demonstrated that the minimum inhibitory concentration and the minimum bactericidal concentration of TP on TH0426 were 1024 and 2048 μg/mL, respectively. After a 4 h treatment, the growth of TH0426 was completely inhibited at the concentration of 1024 and 2048 μg/mL of TP. Meanwhile, TP exhibited a significant antibiofilm activity. Both scanning electron microscope and transmission electron microscope analyses revealed disrupted cell membrane structure, irregular cell morphology, and loss of intracellular contents following TP treatment. Moreover, increased cell membrane permeability induced by TP led to intracellular ion and DNA leakage, resulting in elevated electrical conductivity and DNA extravasation. Furthermore, TP decreased LDH activity, protein concentration and content, DNA fluorescence intensity, and density in a time-dependent manner, indicating inhibition of protein metabolism and DNA synthesis. In conclusion, TP exhibits potent antibacterial properties by inhibiting biofilm formation, disrupting cell membrane integrity, and interfering with protein metabolism and DNA synthesis in drug-resistant A. veronii TH0426 in vitro.

茶多酚对耐药气单胞菌 TH0426 的体外抗菌作用及机制研究
鲟鱼养殖中出现的由鳗鱼气单胞菌(Aeromonas veronii)引起的气单胞菌败血症(MAS)对水产养殖业造成的高死亡率影响已成为一个重大问题。MAS 带来的威胁突出表明,迫切需要采取有效的控制措施来对抗鲟鱼种群中的细菌感染。茶多酚(TP)对畜禽细菌感染有很好的抗菌效果。然而,茶多酚在水生动物细菌性疾病中的抗菌功效和机制在很大程度上仍未得到探索。本研究旨在通过评估 TP 对 TH0426 细胞生长、抗生物膜活性、形态以及电导率、DNA 外渗、乳酸脱氢酶(LDH)活性、蛋白质和 DNA 含量的影响,研究 TP 对鱼类耐药性 A. veronii TH0426 的体外抗菌效果和机制。结果表明,TP 对 TH0426 的最小抑菌浓度和最小杀菌浓度分别为 1024 和 2048 μg/mL。在 1024 和 2048 μg/mL 的 TP 浓度下,经过 4 小时的处理,TH0426 的生长被完全抑制。同时,TP 表现出了显著的抗生物膜活性。扫描电子显微镜和透射电子显微镜分析表明,TP 处理后,细胞膜结构被破坏,细胞形态不规则,细胞内含物丢失。此外,TP 诱导的细胞膜通透性增加导致细胞内离子和 DNA 泄漏,从而导致电导率升高和 DNA 外渗。此外,TP 还能以时间依赖的方式降低 LDH 活性、蛋白质浓度和含量、DNA 荧光强度和密度,表明其抑制了蛋白质代谢和 DNA 合成。总之,TP 通过抑制生物膜的形成、破坏细胞膜的完整性、干扰耐药性 A. veronii TH0426 的蛋白质代谢和 DNA 合成,在体外表现出强大的抗菌特性。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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