Unveiling the Self-assembly and Therapeutic Efficacy of Antimicrobial Peptides SA4 Against Multidrug-Resistant A. baumannii.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Lalita Sharma, Gopal Singh Bisht
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Abstract

Infections linked to Acinetobacter baumannii are one of the main risks of modern medicine. Biofilms formed by A. baumannii due to a protective extracellular polysaccharide matrix make them highly tolerant to conventional antibiotics and raise the possibility of antibiotic resistance. Antimicrobial peptides (AMPs) are gaining popularity due to their broad-spectrum actions and key properties of peptide self-assembly, making them a promising alternative to antibiotics. Here, we demonstrate that 12-residue synthetic self-assembled peptide SA4 nanostructures have enough antibacterial action to prevent the growth of mature bacterial biofilms. The SA4 peptide was successfully synthesized by using the solid-phase peptide synthesis method, and its self-assembly was prepared in water. The self-assembled peptide hydrogel formed nanotube structure was observed under a scanning electron microscope and further characterized to confirm their physical and molecular properties. The resulting hydrogel exhibits significant antibacterial activity against MDR A. baumannii strains (MDR-1 and MDR-2), responsible for many nosocomial infections. In addition, at various gel concentrations, this hydrogel has the potential to inhibit about 30-80% of biofilms formed by MDR strains. Furthermore, under a microscope, it has been observed that the rupture of the bacterial cell membrane and cell wall of A. baumannii cells is caused by peptide nanotubes generated by self-assemblies. Thus, peptide-based nanotubes present intriguing avenues for various biomedical applications. This is the first report of bacterial biofilm removal with SA4 peptide nanotubes, and offering a unique treatment for infections linked to biofilms.

揭示抗菌肽 SA4 的自组装和对耐多药鲍曼尼氏菌的疗效
与鲍曼不动杆菌有关的感染是现代医学的主要风险之一。鲍曼不动杆菌的胞外多糖保护基质形成的生物膜使其对传统抗生素具有很强的耐受性,并有可能产生抗药性。抗菌肽(AMPs)因其广谱的作用和肽自组装的关键特性而越来越受欢迎,使其成为一种有希望替代抗生素的药物。在这里,我们证明了 12 个残基合成的自组装肽 SA4 纳米结构具有足够的抗菌作用,可以阻止成熟细菌生物膜的生长。我们采用固相多肽合成法成功合成了 SA4 肽,并在水中制备了它的自组装。在扫描电子显微镜下观察了自组装多肽水凝胶形成的纳米管结构,并进一步对其物理和分子特性进行了表征。所制备的水凝胶对导致多种院内感染的 MDR 鲍曼尼杆菌菌株(MDR-1 和 MDR-2)具有显著的抗菌活性。此外,在不同的凝胶浓度下,这种水凝胶有可能抑制 30-80% 的 MDR 菌株形成的生物膜。此外,在显微镜下观察到,鲍曼不动杆菌细胞的细菌细胞膜和细胞壁破裂是由肽纳米管自组装产生的。因此,基于肽的纳米管为各种生物医学应用提供了令人感兴趣的途径。这是用 SA4 肽纳米管清除细菌生物膜的首次报道,为治疗与生物膜有关的感染提供了一种独特的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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