Antifungal Susceptibility Testing and Cluster Analysis of Candida auris Strains.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Clinical laboratory Pub Date : 2024-10-01 DOI:10.7754/Clin.Lab.2024.240317

Gulsah E Ozmerdiven, Arzu Irvem, Zeynep Cizmeci

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引用次数: 0

Abstract

Background: Candida auris is an opportunistic pathogen that has become widespread in recent years and shows resistance to multiple drugs. The aim of our study was to determine the antifungal susceptibilities of C. auris isolates, to perform a dendrogram from the mass spectra of strains obtained from matrix-assisted laser desorption ionization-time of flight mass spectrometry in order to evaluate the proteomic similarities of the strains and determine the geographical clade of C. auris strains in this study by the help of Multiplex RT-PCR.

Methods: The samples yielded 58 C. auris isolates. MALDI TOF MS (BioMerieux, France) was used for identification of the isolates and Sensititre Yeast One (Thermoscientific) system was used for antifungal susceptibility testing. Dendrograms of strain's spectra were generated by using the RUO/Saramis (BioMerieux, France) database and evaluated through hierarchical clustering analysis. The selected nine strains were examined at the clade level by using Multiplex RT-PCR. Results The susceptibility profile of the strains revealed resistance to Fluconazole in 84% (MICs ≥ 32) and resistance to Amphotericin B in 60% (MIC ≥ 2). All strains were found to be sensitive to Anidulafungin and Micafungin. The dendrogram of the main spectra of C. auris isolates showed a similarity range of 35 - 100%. The nine strains studied were identified as clade 1 (South Asian).

Conclusions: It was determined that C. auris strains were members of geographical clade 1, and the Amphotericin B resistance was found to be higher than expected. This situation poses a threat to critically ill patients.

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白色念珠菌菌株的抗真菌药敏试验和聚类分析

背景：念珠菌是近年来广泛传播的一种机会性病原体，对多种药物具有抗药性。我们的研究旨在确定白色念珠菌分离株的抗真菌敏感性，通过基质辅助激光解吸电离飞行时间质谱法获得的菌株质谱进行树枝图分析，以评估菌株的蛋白质组相似性，并借助多重 RT-PCR 确定本研究中白色念珠菌菌株的地理支系：方法：样本中分离出 58 株 C. auris。采用 MALDI TOF MS（BioMerieux，法国）对分离菌株进行鉴定，采用 Sensititre Yeast One（Thermoscientific）系统进行抗真菌药敏试验。利用 RUO/Saramis（法国生物梅里埃公司）数据库生成菌株谱树枝图，并通过分层聚类分析进行评估。利用多重 RT-PCR 对所选的 9 个菌株进行了支系水平的检测。结果菌株的药敏谱显示，84%的菌株对氟康唑耐药（MIC ≥ 32），60%的菌株对两性霉素 B 耐药（MIC ≥ 2）。所有菌株均对阿尼芬灵和米卡芬灵敏感。肛门癣菌分离物主光谱的树枝状图显示，相似度范围为 35 - 100%。研究的 9 株菌株被确定为支系 1（南亚）：结论：已确定 C. auris 菌株是地理支系 1 的成员，且两性霉素 B 的耐药性高于预期。这种情况对重症患者构成了威胁。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.