Functional observational battery (FOB) tests using caffeine and chlorpromazine hydrochloride in sprague-dawley rats.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-10-08 DOI:10.14715/cmb/2024.70.9.2

Subramanian Baskaran, Prakash Malaiarasan, Sanjaykumar Mansukhlal Paneliya

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Abstract

Caffeine is believed to exert its therapeutic effects by acting as a nonselective, competitive antagonist of adenosine receptors. Chlorpromazine, a phenothiazine, is a classic psychotropic mediator extensively used in the clinical administration of psychotic disorders. This study aimed to validate the procedures used for performing Functional Observational Battery (FOB) tests, to demonstrate the proficiency and interobserver reliability during the FOB tests and also to assess effect on neurobehavioral parameters using positive controls in rats. The rats were administered with Caffeine in Milli-Q water as oral gavage at the dose of 20 mg/kg and Chlorpromazine HCl in 0.9% Saline as intraperitoneal route at the dose of 20 mg/kg. No inter-personnel variability was observed in home cage, handling, open field and sensory reactivity observations recorded in Proficiency test. In conclusion, the known effects of positive controls; caffeine and chlorpromazine HCl on neurobehavioral/Functional Observational Battery parameters including autonomic, neuromuscular and sensory reactivity tests were detected in the current study. FOB test procedures for neurobehavioral, grip strength and motor activity are adequate for the detection of neurotoxic effects of positive controls. No major inter-personnel variability was observed between study personnel in neurobehavioural observations.

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使用咖啡因和盐酸氯丙嗪对布拉格道利大鼠进行功能观察电池（FOB）测试。

咖啡因被认为是通过作为腺苷受体的非选择性竞争性拮抗剂而发挥治疗作用的。氯丙嗪是一种吩噻嗪类药物，是一种典型的精神药物，广泛用于临床治疗精神病。本研究旨在验证用于进行功能观察电池（FOB）测试的程序，证明 FOB 测试的熟练程度和观察者之间的可靠性，并使用阳性对照组评估对大鼠神经行为参数的影响。大鼠口服的剂量为 20 毫克/千克毫升水溶液中的咖啡因，腹腔注射的剂量为 20 毫克/千克 0.9% 生理盐水中的盐酸氯丙嗪。在能力测试中记录的家笼、操作、露天场地和感官反应性观察结果中，没有观察到人与人之间的差异。总之，在本研究中检测到了阳性对照组咖啡因和盐酸氯丙嗪对神经行为/功能观察电池参数（包括自主神经、神经肌肉和感觉反应测试）的已知影响。神经行为、握力和运动活动的功能观察电池测试程序足以检测阳性对照组的神经毒性效应。在神经行为观察方面，未发现研究人员之间存在重大差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.